There is a known HindIII site off-center in the 3 kb insert.It is 2 kb downstream from one end (end A), and 1 kb downstream from the other end (end B).
There is a known HindIII site 1 kb off-center in the 3 kb insert. Meaning its 2 kb downstream from one end (end A), and 1 kb downstream from the other end (end B).