PCR amplification eventually plateaus after an uncertain number of cycles; low initial concentrations of target nucleic acid molecules may not amplify to detectable levels; and PCR amplification efficiency in a sample of interest may be different from that of reference samples.
PCR amplification eventually plateaus after an uncertain number of cycles; and low initial concentrations of target nucleic acid molecules may not amplify to detectable levels. However, the most significant limitation of PCR is that PCR amplification efficiency in a sample of interest may be different from that of reference samples.