Difference between revisions of "User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/16"

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Objective

We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol

Description

1. Add experimental record here. Include what, how, and why...

Data

Stock Solution

1. Buffer
1. 0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM
2. Luminol
1. Dissolve 12.9mg luminol in 300uL of DMSO
2. Add to 50mL buffer ---> 1.46mM
3. H2O2
1. 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM
2. Check absorption at 250 source (ε(250) = 16.69 M-1cm-1). A = 0.7392. [H2O2] = 44.29mM

Notes

- We measured the absorbance of the 1/20 luminol to see if it still absorbed. We found the absorbance peak to be less than 1.00, specifically around 0.300

Graph showing the absorbance spectra for citrate AuNP and HRP AuNP

Making the BSA AuNP solution Add 1mL of the 2.54mM gold (HAuCl4) solution to a 10mL volumetric flask Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA

```   - Calculations = ((0.00254 moles/L Gold)* (1L/1000ml)* (1ml)) / 90 = moles BSA = 2.82*10^-8 moles BSA
[BSA] = 15.6μM
- (2.82*10^-8 moles) * (1L/15.6*10^-6 moles) = 0.00180L or 1.8mL BSA to add to solution
```

Add deionized water up to 10mL Transfer solution to a test tube and cap with aluminum foil Heat in oven at 80C for 3 hours
The text passage contains a scientific experiment record, including the preparation of stock solutions and a calculation for making a BSA AuNP solution.
single_question_with_answer

the passage includes a calculation that serves as a question (how much bsa to add to achieve a specific concentration) and provides the answer (1.8 ml bsa).
Given a text passage, determine:
    1. If it contains a mathematical question (including word problems or calculations)
    2. If an answer to that mathematical question is provided in the text

First, describe the content structure you found in 1-2 sentences.

Then provide your analysis in the following format:
TYPE: <one of the following values>
- NO_QUESTION
- SINGLE_QUESTION
- SINGLE_QUESTION_WITH_ANSWER
- MULTIPLE_QUESTIONS
- MULTIPLE_QUESTIONS_WITH_ANSWERS
system
Difference between revisions of "User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/10/16"

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

We are going to test the activity of our HRP-NPs today for the catalytic conversion of luminol

Description

1. Add experimental record here. Include what, how, and why...

Data

Stock Solution

1. Buffer
1. 0.6140g Tris in 1L, pH set to 8 with HCl ---> 5.1mM
2. Luminol
1. Dissolve 12.9mg luminol in 300uL of DMSO
2. Add to 50mL buffer ---> 1.46mM
3. H2O2
1. 312uL 30% H2O2 into 100mL buffer ---> Should be 45mM
2. Check absorption at 250 source (ε(250) = 16.69 M-1cm-1). A = 0.7392. [H2O2] = 44.29mM

Notes

- We measured the absorbance of the 1/20 luminol to see if it still absorbed. We found the absorbance peak to be less than 1.00, specifically around 0.300

Graph showing the absorbance spectra for citrate AuNP and HRP AuNP

Making the BSA AuNP solution Add 1mL of the 2.54mM gold (HAuCl4) solution to a 10mL volumetric flask Add an appropriate amount of BSA solution so that the final concentration of gold is 90X that of BSA

```   - Calculations = ((0.00254 moles/L Gold)* (1L/1000ml)* (1ml)) / 90 = moles BSA = 2.82*10^-8 moles BSA
[BSA] = 15.6μM
- (2.82*10^-8 moles) * (1L/15.6*10^-6 moles) = 0.00180L or 1.8mL BSA to add to solution
```

Add deionized water up to 10mL Transfer solution to a test tube and cap with aluminum foil Heat in oven at 80C for 3 hours
user
The text passage contains a scientific experiment record, including the preparation of stock solutions and a calculation for making a BSA AuNP solution.

TYPE: SINGLE_QUESTION_WITH_ANSWER

The passage includes a calculation that serves as a question (how much BSA to add to achieve a specific concentration) and provides the answer (1.8 mL BSA).