HIGH AFFINITY, DSRNA BINDING BY DISCONNECTED INTERACTING PROTEIN 1\xe2\x80\xa0
Disconnected Interacting Protein 1 (DIP1) appears from sequence analysis and preliminary binding studies to be a member of the dsRNA-binding protein family. Of interest, DIP1 was shown previously to interact with and influence multiple proteins involved in transcription regulation in Drosophila melanogaster. We show here that the longest isoform of this protein, DIP1-c, exhibits a 500-fold preference for dsRNA over dsDNA of similar nucleotide sequence. Further, DIP1-c demonstrated very high affinity for a subset of dsRNA ligands, with binding in the picomolar range for VA1 RNA and miR-iab-4 precursor stem-loop, a potential physiological RNA target involved in regulating expression of its protein partner, Ultrabithorax.
<!>Expression and Purification of DIP1-c<!>Magnetic Circular Dichroism<!>Nucleic acid preparation and handling<!>DNA Templates<!>Folding RNA<!>Radiolabeling<!>Gel Retardation<!>Determining DIP1-c Protein Absorption Coefficient<!>DIP1-c High Affinity Binding to VA1 RNA<!>DIP1-c Binding to dsRNA and dsDNA<!>DIP1-c Binds with High Affinity to a MicroRNA Precursor Stem-Loop<!>DISCUSSION<!>
<p>A subset of RNA binding proteins can bind double-stranded RNA (dsRNA)1 and function in splicing, RNA localization, translational control, RNA editing, post-transcriptional gene silencing, and cellular defense (reviewed in [1,2]). These proteins recognize and bind dsRNA through one or more dsRNA-binding domains (dsRBDs), which are 65–68 amino acids in length [3]. Proteins with dsRBDs bind similar dsRNA ligands with comparable affinity in vitro, generally in the nanomolar range [4–6]. Since most proteins in this family contain multiple dsRBDs, specificity for a particular dsRNA molecule is thought to arise from the spatial arrangement of multiple domains rather than activity of a single domain [7]. The sequences of dsRBDs are loosely conserved and can be divided into two types: Type A domains bind dsRNA with higher affinity (nanomolar range), and Type B domains generally bind dsRNA weakly [3,7].</p><p>Disconnected Interacting Protein 1 (DIP1) contains two dsRBDs based on sequence similarity [8,9]. Interestingly, the closest sequence identity for DIP1 is found within the dsRBD sequences of RNA editase enzymes [8]. The first dsRBD is predicted to be a Type A domain, whereas the second dsRBD is a Type B [8,9]; however, isolated dsRBD1 does not bind dsRNA [9]. These results are inconsistent with the prevailing view that Type A domains bind dsRNA tightly, whereas Type B domains are for cooperative binding and/or to promote protein dimerization [10,11].</p><p>DIP1 was previously identified as a protein partner of Ultrabithorax (Ubx) [8], Disconnected [9], and Suppressor of variegation 3–9 [12], suggesting involvement in transcription regulation [8,9] and chromatin remodeling [12]. Indeed, we have shown that DIP1-c, the longest isoform, blocks transcription activation by Ubx using a yeast one-hybrid assay [8]. Although this function is not common for dsRNA-binding proteins, the dsRBDs in DIP1-c appear to be active, as shown in Northwestern blots of full-length protein [8] and the individual dsRBDs [9]. DIP1-c preferentially binds dsRNA over ssRNA and dsDNA [8], which is similar to other proteins with dsRBDs.</p><p>DIP1-c demonstrates unusual sequence selectivity based on its higher affinity for the adenovirus VA1 RNA versus the HIV TAR stem-loop RNA, both highly-structured RNA ligands, with remarkably high affinity for VA1 RNA, ~50 pM [8]. The highest affinity for dsRNA previously was demonstrated by XlrbpA [13]. Although the basis of higher affinity association by DIP1-c is unknown, the observation suggests that dsRBDs in DIP1-c might discriminate among different ligands. In the present work, we examine further the dsRNA-binding behavior of DIP1-c and identify its high affinity for an interesting Drosophila microRNA precursor stem-loop sequence [14,15].</p><!><p>Expression and purification of DIP1-c has been described [8]. Given that the histidine tag was shown not to alter the affinity of DIP1-c for dsRNA [8], His6-DIP1-c was used for these experiments. DIP1-c was dialyzed into 50 mM NaH2PO4, pH 8.0, 300 mM NaCl, 5% glucose, ~5 mM DTT following the Ni+2-nitrilotriacetic acid-agarose chromatography step [8].</p><!><p>Magnetic circular dichroism was used to determine the absorption coefficient of DIP1-c at 280 nm. N-acetyl-tryptophanamide (NATA, Aldrich) was used as a standard. Multiple DIP1-c samples were dialyzed in either a) 25 mM NaH2PO4, pH 7.5, 150 mM NaCl, 2% glucose or b) 50 mM NaH2PO4, pH 8.0, 150 mM NaCl, 5% glucose. All samples were analyzed from 280–320 nm on a J-500C Spectropolariometer equipped with a 7020A power supply and UV-visible photomultiplier tube (Japan Spectroscopic Co.). Standard curves for NATA utilized the difference in values between the 292 nm peak and 320 nm plateau. Using the tryptophan concentration determined for DIP-c from this method allowed calculation of its molar absorption coefficient at 280 nm.</p><!><p>dsDNA40: Oligonucleotides bearing the sequence 5′-CCGGGCTGCACATGGTTAATGGCCAGTCCACGCGTAGATC-3′ and its complement were annealed, radiolabeled, and utilized in gel retardation as described below for RNA. All RNA ligands were transcribed and dephosphorylated in vitro as described [8] with few modifications.</p><!><p>All templates, except where noted, were synthesized oligonucleotides (Biosource, Inc.) with the appropriate sequence downstream of the T7 RNAP promoter annealed to the T7 RNAP promoter primer [8]. Adenovirus VA1 RNA: Full-length VA1 was synthesized as described [8]. VA1 20–108 template was produced by PCR amplification of bases 20–108 of full-length VA1. Other deletions of the VA1 20–108 sequence were synthesized oligonucleotides. dsRNA40: The templates were identical to dsDNA40 described above except the 40 bp sequences were preceded by 5′-GGGAGA-3′, a sequence that promotes high yield using T7 RNAP [16]. 25dsRNA-4Loop: An oligonucleotide bearing the sequence 5′-GGATCTCATCATGGCGGACGTACAG-3′ and its complement were used to produce a 25 bp hairpin with the tetraloop 5′-ACUG-3′. miR-iab-4 stem-loop: The template bearing the sequence 5′-TCGTAAACGTATACTGAATGTATCCTGAGTGTATCCTATCCGGTATACCTTC AGTATACGTAACACGA-3′ (miRBase, [17]), was synthesized as an oligonucleotide.</p><!><p>RNA was diluted to 1 µM or 10 nM in 20 mM Tris-HCl, pH 7.5, 100 mM KCl, and was heated to >95°C and allowed to cool slowly. At 65°C, a final concentration of 10 mM MgCl2 was added. RNA was either allowed to cool slowly to room temperature or immediately placed on ice.</p><!><p>RNA was radiolabeled as described [8], except the kinase reaction buffer was 40 mM Tris-HCl, pH 8.0, 10 mM MgCl2, 0.4 mM spermidine, 5% glycerol, 5 mM DTT, and the NICK™ gel filtration column (GE Healthcare) running buffer was 20 mM Tris-HCl, pH 7.5, 100 mM KCl, 1 mM MgCl2.</p><!><p>Gel retardation assays were performed as described [8]. Stoichiometric measurements were performed with nucleic acid concentrations in the nanomolar range, whereas the nucleic acid concentration for binding affinity assays was in the sub-picomolar range. Binding was measured in 20 mM Tris-HCl, pH 7.5, 100 mM KCl, 1 mM MgCl2, 100 µg/mL Bovine Serum Albumin, 5 mM DTT, 6.7% or 10% glycerol. All reactions involving VA1 RNAs, dsRNA40 and dsDNA40 were performed with buffer containing 10% glycerol; miR-iab-4 and 25dsRNA-4Loop reactions were performed in buffer containing 6.7% glycerol. DIP1-c was present in the appropriate concentration range. Data were acquired using a Fuji phosphorimager FLA-5000 and imaging software, ImageGuage 4.0 and MultiGuage 2.3 (Fuji Photo Film Co., Ltd., Tokyo, Japan). Bound nucleic acid data were analyzed with Igor Pro version 4.02A (Wavemetrics, CA) and were fit to the equation:
Y=Ymax([DIP1]n[DIP1]n+Kdn)+b
Free nucleic acid data were fit to the equation:
Y=Ymax−[Ymax([DIP1]n[DIP1]n+Kdn)]+b
where Y (fraction bound or free) varies, Ymax is the maximum bound dsRNA, Kd is the equilibrium dissociation constant, n is the Hill coefficient and was allowed to float, and b is the background radioactivity in the absence of protein. Where Kd values for bound and free data differed, free data were used to avoid distortions from artifacts of complex dissociation during gel electrophoresis. All Kd values were adjusted for activity from stoichiometric measurements at conditions of dsRNA ligand concentration ≫Kd and were then averaged. The standard deviation was calculated for multiple experiments that encompassed at least two separate DIP1-c purifications.</p><!><p>Our laboratory has previously shown that DIP1-c, the longest isoform, preferentially binds dsRNA over ssRNA, and its affinity for structured RNA ligands, such as the adenovirus VA1 RNA, is >1000-fold higher than for dsDNA [8]. To perform a more detailed analysis of the RNA binding behavior of DIP1-c, the absorption coefficient for this protein was established to allow precise definition of protein concentration and hence, dissociation constants. Magnetic circular dichroism was used to measure the precise concentration of tryptophan [18] in a DIP1-c solution, which was used to calculate an extinction coefficient of 33,600 ± 4000 M−1cm−1 at 280 nm.</p><!><p>We have previously demonstrated DIP1-c binding to HIV TAR RNA and adenovirus VA1 RNA, with the latter having higher affinity (Kd = 45 ± 11 pM) (Figure 1D) [8]. To explore the key elements for binding, we dissected the VA1 RNA structure. The binding footprint for dsRBDs has been reported to be a minimum of 11 bp [20,21] or as large as 18–20 bp [13]. The secondary structure of full-length VA1 RNA, predicted using an online algorithm [19], identifies only one region in the structure that satisfies the 11 bp criterion (Figure 1A, arrows).</p><p>Encompassing bases 20–108 of full-length VA1 RNA, the "long arm" was tested for DIP1-c binding. DIP1 maintained high affinity for this truncated VA1 RNA (Kd = 100 ± 27 pM) (Figure 1D). Systematic removal of a third of the structure on either end of the long arm (Upper 2/3 or Lower 2/3) resulted in significant decreases in affinity (Figure 1D). To test the middle region of the VA1 long arm, we transcribed the middle 13 base pair duplex within a 25 base pair hairpin (25dsRNA-4Loop) (Figure 1B). DIP1-c bound 25dsRNA-4Loop with nanomolar affinity (Figure 1D). These binding parameters for DIP1-c are within the reported range of other proteins with dsRBDs [4–6]. DIP1-c associates nonspecifically with the Lower 1/3 of the long arm of VA1 RNA (Figure 1D). From these results, we conclude that elements beyond the extended dsRNA region of the VA1 long arm are important for higher affinity binding.</p><!><p>To compare dsRNA and DNA binding, an RNA duplex corresponding to a DNA duplex was transcribed in vitro with six base overhangs on each 5′ end (dsRNA40) (Figure 2A). DIP1-c binds dsRNA40 with very high affinity (Kd = 55 ± 31 pM) (Figure 2C). As anticipated for this protein, a DNA duplex of the same sequence (dsDNA40) is bound ~500-fold more weakly by DIP1-c (Kd = 26 ± 8 nM) (Figure 2C). The higher affinity exhibited by DIP1-c for dsRNA40 contrasts the lower affinity measured for 25dsRNA-4Loop, an RNA hairpin of only 25 bp (Figure 1C). This observation suggests one factor that promotes higher affinity binding to VA1 RNA may be the length of the double-stranded segment of the long arm (bases 20–108).</p><!><p>Based on the observation that DIP1 interacts with multiple proteins [8,9,12], including the Hox protein Ubx, multiple physiological RNA targets may exist. We therefore examined potential RNA targets associated with Ubx. There is growing evidence that miRNAs play important roles in the complex regulation of Hox genes [22,23]. Within this category of targets [24–26], one miRNA, miR-iab-4-5p, directly inhibits Ubx activity in vivo [22]. Further, iab-4 is a genomic element that affects Hox gene expression, including ubx, as a long-range enhancer [14,15]. With the potential for DIP1 to interact with the products of long-range enhancers and simultaneously with transcription factors bound to relevant promoter regions, we examined DIP1-c binding to the miR-iab-4 precursor stem-loop and found very tight binding, Kd = 45 ± 11 pM (Figure 3C).</p><!><p>dsRNA-binding domains are reported to recognize and bind through interactions with the sugar-phosphate backbone [3,27], although contacts between dsRBDs and ssRNA regions within a target have been observed by NMR [28,29]. The number of physiological dsRNA targets identified for proteins with dsRBDs is increasingly important [e.g., 30–34]. Proteins with dsRBDs, which are presumed to bind nonspecifically, must possess mechanisms for recognizing targets among all RNA species in vivo [2], but the basis for this selectivity remains elusive. DIP1-c was isolated as a protein partner for an interesting set of proteins: a Hox transcription factor, (Ultrabithorax [8]), a zinc-finger transcription factor (Disconnected [9]), and a histone methyltransferase (Suppressor of variegation 3–9 [12]). DIP1-c, like other dsRNA-binding proteins, preferentially binds dsRNA tighter than ssRNA or dsDNA [8], and here, we have demonstrated a 500-fold preference for dsRNA over dsDNA of similar sequence.</p><p>Standard ligands to assess dsRNA binding include the adenovirus VA1, HIV TAR, and poly(I):poly(C) dsRNA [4–6], despite their apparent lack of physiological relevance. dsRNA-binding proteins generally bind these dsRNAs with affinities in the micromolar to nanomolar range [4–6]. Interestingly, DIP1-c binds to dsRNA in the picomolar range, with the highest affinity for dsRNA currently reported in the literature (although dsRNA-binding proteins may bind their physiological targets with higher affinity). The identification of physiological RNA ligands for many dsRNA-binding proteins remains largely elusive. The length of the duplex may play a role in higher affinity binding, although sequence specificity has not been ruled out [28]. A dependence on the RNA duplex length has been observed for XlrbpA binding [13] and PKR binding and activity [35,36]; however, the ligands tested were perfect duplexes and of unknown physiological relevance.</p><p>DIP1-c demonstrates both high affinity and binding preferences on both non-physiologically- and potentially physiologically-relevant ligands. VA1 RNA is highly structured, but possesses only one region that satisfies the criterion of at least 11 contiguous base pairs for the dsRBD binding footprint [20,21]. Truncation of VA1 RNA weakens DIP1-c affinity to the nanomolar range, provided the perfect 13 bp region is present. Thus, DIP1-c recognizes the A-form helix of dsRNA, but other features of the VA1 RNA must contribute to higher affinity binding.</p><p>To examine binding to an interesting physiological dsRNA, we selected a target associated with the DIP1-c protein partner Ubx. Although six microRNAs are computationally predicted to target ubx mRNA [24–26], only one, miR-iab-4-5p, has been shown to directly inhibit Ubx activity in vivo [22]. This miRNA is produced by the cleavage of a stem-loop within the transcribed iab-4 long-range enhancer [37]. DIP1-c binds with remarkably high affinity to the miR-iab-4 stem-loop (Kd ~50 pM), comparable to its affinity for full-length VA1 and dsRNA40. The similarity in miR-iab-4 and VA1 long arm RNA structures and the higher affinity for these RNAs suggests the potential for specific features (e.g., loops, bulges, kinks, bubbles, and spatial arrangements of multiple duplex segments) to promote high-affinity binding.</p><p>DIP1 is a remarkably multi-functional protein with the potential for binding both dsRNA and multiple proteins, a duality that allows recruiting one of its partner proteins along with a specific RNA ligand. This multi-ligand binding suggests the potential for DIP1 to serve as a "bridge." The studies presented here open new directions for investigations of dsRBD selection and impact on other functional aspects of DIP1. Identification of in vivo RNA targets will provide new avenues to explore the physiological consequences of DIP1 binding to these RNAs and its protein partners.</p><!><p>This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.</p><p>Supported by grants from the Robert A. Welch Foundation (C-576) and the National Institutes of Health (GM22441) to KSM. DJC was trained on the NIH Houston Area Molecular Biophysics Predoctoral Training Grant 5T32-GM08208.</p><p>ABBREVIATIONS:</p><p>DIP1, Disconnected Interacting Protein 1</p><p>DIP1-c, longest DIP1 isoform</p><p>dsRBD, double-stranded RNA-binding domain</p><p>DTT, dithiothreitol</p><p>HIV, human immunodeficiency virus</p><p>Kd, equilibrium dissociation constant</p><p>miRNA, microRNA</p><p>NATA, N-acetyl-tryptophanamide</p><p>RNAP, RNA Polymerase</p><p>Ubx, Ultrabithorax</p>