Molecular basis of intramolecular electron transfer in proteins during radical-mediated oxidations: Computer simulation studies in model tyrosine-cysteine peptides in solution
Experimental studies in hemeproteins and model Tyr/Cys-containing peptides exposed to oxidizing and nitrating species suggest that intramolecular electron transfer (IET) between tyrosyl radicals (Tyr-O\xe2\x97\x8f) and Cys residues controls oxidative modification yields. The molecular basis of this IET process is not sufficiently understood with structural atomic detail. Herein, we analyzed using molecular dynamics and quantum mechanics-based computational calculations, mechanistic possibilities for the radical transfer reaction in Tyr/Cys-containing peptides in solution and correlated them with existing experimental data. Our results support that Tyr-O\xe2\x97\x8f to Cys radical transfer is mediated by an acid/base equilibrium that involves deprotonation of Cys to form the thiolate, followed by a likely rate-limiting transfer process to yield cysteinyl radical and a Tyr phenolate; proton uptake by Tyr completes the reaction. Both, the pKa values of the Tyr phenol and Cys thiol groups and the energetic and kinetics of the reversible IET are revealed as key physico-chemical factors. The proposed mechanism constitutes a case of sequential, acid/base equilibrium-dependent and solvent-mediated, proton-coupled electron transfer and explains the dependency of oxidative yields in Tyr/Cys peptides as a function of the number of alanine spacers. These findings contribute to explain oxidative modifications in proteins that contain sequence and/or spatially close Tyr-Cys residues.
Introduction<!>System setup, parameters and molecular dynamic (MD) simulations<!>Thermodynamic integration<!>Quantum mechanics (QM)-based calculations<!>ET coupling matrix elements calculation using the Pathways algorithm<!>Results<!>Overall reaction<!>Mechanism A<!>Mechanism B<!>Mechanism C<!>Mechanism D<!>ET kinetics<!>Previous results on ET between Tyr and Cys residues<!>How does the radical transfer reaction occur in Tyr/Cys-containing peptides in aqueous solution?<!>Conclusions
<p>Tyrosine (Tyr) and cysteine (Cys) are key target residues in proteins for free radical-dependent post-translational modifications associated to oxidative stress conditions (1). Indeed, both amino acids can be oxidized by one-electron oxidants such as hydroxyl radical (●OH), nitrogen dioxide (●NO2), carbonate radical (CO3●−), peroxyl radicals (ROO●) and oxoferryl complexes to yield the corresponding amino acid-derived radicals (2-5), namely tyrosyl (Tyr-O●) and cysteinyl (Cys-S●) radicals, respectively. In proteins, Tyr-O● and Cys-S● are transient species and typically evolve to a variety of products including, 3,3′-dityrosine, 3-nitrotyrosine, cysteine disulfide, S-nitrosocysteine and cysteine sulfinic acid (1, 6) and/or participate in inter- or intra-molecular electron transfer (IET) processes (7-12) as schematically shown in Figure 1.</p><p>In most cases only a few residues in proteins are oxidatively modified in vivo by free radical reactions, although the molecular bases of the selectivity are far from being understood (13, 14). In this regard, in spite of the initial sites of free radical attack in a protein, the formation and relative stability (or half-life) of Tyr-O● and Cys-S● seem to represent key factors controlling the final sites and yields of protein oxidative modifications. Particularly important is the fact that in peptides (8-10, 15, 16) and proteins (17-20) electron transfer processes (either inter or intramolecular) involving tyrosine and/or cysteine residues have been observed. Early work has shown that the redox potential for theTyr/Tyr-O● and Cys/Cys-S● couples are quite close: tyrosine has a one-electron redox potential value of 0.93-0.94 V at pH 7.0 and 25°C, while that of cysteine is slightly higher (i.e. ΔE°<+10 mV at pH 7.0) (10, 21-23). Similarly, the Keq for the one-electron oxidation reaction of GSH by Tyr-O● at pH 7.15 has been recently reported to be close to one (24). Nonetheless, the one-electron redox potentials of the amino acids such as Tyr and Cys within proteins can significantly differ, depending on factors that include local structure and solvent accessibility, among others (21, 25, 26).</p><p>The presence of a Cys residue in the proximity of a Tyr residue has been reported to facilitate radical transfer in a way that Cys usually acts as a radical sink. For instance, hydrogen peroxide-mediated oxidation of myoglobin produces Cys100 thiyl radicals via an intermolecular electron transfer from Cys110 to Tyr103-phenoxyl radicals of another myoglobin molecule, ultimately yielding homodimers with disulfide bond formation (18, 19); similarly, peroxynitrite-mediated oxyhemoglobin oxidation results in the formation of thiyl radicals at Cys93 with the intermediacy of Tyr42 and Tyr24 phenoxyl radicals (19), and the formation of disulfide and dityrosine intermolecular cross-links (17). The role of Tyr-Cys IET in ribonucleotide reductase catalysis has been largely explored and established (20). Indeed, experimental studies performed in model peptides in aqueous solution, containing both Cys and Tyr residues suggest that rapid IET between the Tyr-O● and Cys residue controls the extent of Tyr nitration, with concomitant Cys oxidation and/or nitrosation, indicating the presence of a Cys-S● radical intermediate (16). These results were also related to the fact that many reported S-nitrosated mitochondrial proteins, share a Tyr and Cys sequence (27), highlighting the importance of the IET between both residues. A first insight into the modulation of tyrosine nitration by cysteine via IET mechanisms was determined by comparing the nitration yield of YAnC peptides with increasing amount of Ala spacers, which showed a decrease in the yield of nitrated peptides with increasing number of Ala spacers (16). The IET reaction between Tyr and Cys residues in the same peptide can potentially occur through different mechanisms which at the atomic level could be described as A, B, C and D in Figure 2.</p><p>Overall, the forward reaction consists of the transfer of the radical moiety from to the Cys residue, resulting in Cys-S● and an unmodified Tyr residue (Reaction 1). The general reaction could be described as either a hydrogen atom transfer (HAT), or a proton-coupled electron transfer (PCET) as defined in its broadest sense following Hammes-Shiffer and Soudackov́s work (28) (i.e. involving movement of a proton and an electron through different bonds in either a time-concerted or sequential manner):
[1]TyrO●−Cys⇋Tyr−CysS●
Briefly, in Mechanism A the reaction is proposed to occur through a direct hydrogen-(●H), or time-concerted PCET directly from the Cys residue towards the Tyr-O●, which relies on the possible intramolecular hydrogen bond (HB) formation between Tyr and Cys residues to transfer the hydrogen or proton. Mechanism B involves a concerted IET, but with a proton transfer through a bridging water molecule, while in Mechanism C, IET occurs first, reducing the Tyr-O● radical to phenolate (Tyr-O−), with concomitant Cys oxidation, which later results in proton release. Finally, Mechanism D, involves the proton and IET steps significantly separated in time, resulting in an acid-base equilibrium-dependent three step reaction involving Cys deprotonation, IET in the charged peptide, and TyrO− protonation (Fig. 2, referred as Step 1D, 2D and 3D, respectively).</p><p>All the involved reactions can be reversible and display different thermodynamic and and kinetic properties that could be affected by the relative position of Tyr and Cys residues within the protein and their environment.</p><p>In this work we have used molecular-based computer simulation techniques (molecular dynamics and quantum mechanics-based calculations) to study the possible mechanisms of radical transfer (or IET) involving tyrosyl and cysteinyl residues in model peptides at the atomic level. To this end, we have analyzed each of the four mechanisms presented above in four peptides containing Tyr and Cys residues separated by different number of Ala spacers in explicit water, that allow to have a direct comparison with recent experimental data. Our results show that the radical transfer reaction is most likely to occur by Mechanism D, which involves an acid/base equilibrium, followed by a fast IET that can be described as a solvent-mediated, PCET reaction</p><!><p>For the present work we firstly built four different size peptides, in each of five possible charge or protonation states, corresponding to: TyrOH-(Ala)n-CysSH, TyrO●-(Ala)n-CysSH, TyrOH-(Ala)n-CysS●, TyrO●-(Ala)n-CysS− and TyrO−-(Ala)n-CysS● for n = 0, 1, 2 and 4. The amino terminal of all peptides was capped with an acetic acid and the carboxy terminal with a methylamine, through the corresponding peptide bonds. Classical molecular dynamics simulations for the peptides were performed in explicit solvent using the TIP3P water model. The simulated water box had a volume of ca. 65 nm3 and between 800 and 1,500 water molecules. All MD simulations were performed using the AMBER force field with the PARM99 set of parameters (29). The point charges for Tyr-O● and Tyr-O− were obtained using the restricted electrostatic potential (RESP) formalism (30) with the optimized structures computed with HF/6-31G* using the Gaussian-03 program (31). The same procedure was applied to determine point charges for Cys-S● and negatively charged Cys-S− residues.</p><p>All simulations were performed using the Periodic Boundary Conditions approximation and the Particle Mesh Ewald (PME) summation method with a grid spacing of 1 Å for treating long range electrostatic interactions, while a direct cut-off distance of 10 Å was used for direct interactions. The Shake method was used to constraint the H atoms at their equilibrium distance allowing the use of 2 fs time step. The Berendsen thermostat was used to keep the temperature constant at 300 K (32). All MD simulations were performed with the AMBER program package (33). The equilibration protocol for all peptides consisted of heating the optimized structures from 0 to 300 K gently in 0.12 ns, while the volume of the systems was kept constant. Then, constant isotropic pressure simulations were performed for 0.08 ns followed by other 12 ns of simulation at constant pressure.</p><!><p>Thermodynamic Integration (TI) scheme (32) as implemented in the AMBER packages (33) was used for computing the Gibbs free energy differences for the following reactions: i) The change in the solvation Gibbs free energy difference for the overall reaction (Reaction 1) and ii) The change in the solvation Gibbs free energy for the charge transfer reaction in the charged peptides (Step 2D). The method consists in slightly changing the system (in this case the peptide) as defined by the classical force field parameters from those corresponding to the initial, to the final state. The change is performed by varying the coupling parameter ζ in eleven discrete steps, from ζ =0 to ζ =1. For each reaction, 11 windows corresponding to ζ values of 0.01, 0.1, 0,2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 0.99 were simulated, and for each one at least a 2ns long MD was performed, or until convergence of the corresponding <δE/δζ> value for each window. The Gibbs free energy was then obtained by numerically integrating the <δE/δζ>vs ζ curve.</p><!><p>QM calculations were performed in vacuum and in implicit solvent with the B3LYP(34, 35) functional and 6-31G* basis set using the Gaussian-03 program (31). Compared to other density functional methods, B3LYP has shown good performance for the study of intramolecular addition of Cys-S● to Phe and Tyr residues (35). The different steps of the catalytic cycle of cytochrome oxidase, which include electron and proton or hydrogen transfer reactions have also been successfully studied using this level of theory (34, 36-38), while the experience using Density Functional Theory methods for PCET in metallo-enzymes was recently reviewed (39). The implicit solvation methods correspond to the Polarizable Continuum Model (PCM) as developed in (40). All QM calculations were performed only for the corresponding peptides in vacuum. For selected cases, QM calculations were also performed with the explicit inclusion of a small number of water molecules in the system. In all cases no significant differences were obtained in the observed trends. Given the known drawbacks (41) concerning the use of implicit solvation methods, especially when direct interactions with water molecules are present as in the case of charged peptides, for several reactions we also computed the change in the solvation Gibbs free energy along the reaction using classical explicit solvation methods and TI scheme as described above. These methodologies have the advantage of explicitly taking into account the solute solvent interaction and the entropy associated with water reorganization (42).</p><!><p>According to Marcus theory, the ET rate (kET) depends on the coupling matrix between donor and acceptor TDA, the reorganization energy (λ) and the reaction Gibbs free energy (ΔG°) as described by the following equation (43):
(Equation 1)kET=2π∕h∣TDA∣2exp(−λ+ΔG∘)2∕4πλkBT∕√(4πλkBT)</p><p>The electronic coupling matrix, usually referred as TDAbetween donor and acceptor for the TyrO●−(Ala)n-CysS- and TyrO−-(Ala)n-CysS● peptides were estimated using the pathways algorithm developed by Beratan et al. (44, 45) using the default parameters. The TDA is related to the probability of ET transfer and directly involved in the resulting ET rate, as described by Marcus theory. Briefly, the pathways method looks for the best possible path, the one with the highest TDA, that connects donor and acceptor selected orbitals. Orbitals are defined as either located on covalent bonds, or in free electron pairs as in oxygen atoms. This results, for example, in a water molecule displaying four orbitals with tetrahedral geometry, two corresponding to the O-H bonds and two corresponding to the free electron pairs. TDA for a given pathway is computed as the product of a number of steps, each with a given coupling value, that define the pathway. A step can either be through atom, when two connected orbitals share the central atom, which are assumed to have a coupling value of 0.6, or through space steps (or jumps) connecting orbitals separated by empty space, for which the coupling is assumed to decay exponentially with the orbital to orbital distance with a decay factor (β=−1.7). As a result of the different structures and ET paths, ET rates can vary as much as two orders of magnitude for the same donor acceptor distance. The TDA was computed between the Tyr-O and Cys-S located orbitals for a hundred snapshots taken from the corresponding peptide MD simulation. For each case, the average TDA along the whole MD is reported. Detailed visual inspection of the ET path allowed determination of the structural type of path involved.</p><!><p>The results are organized as follows: first, the thermodynamics of the overall radical transfer reaction is studied in vacuum and in water (Reaction 1), for each of the different Ala spacer-containing peptides. Then, all steps for each possible mechanism are comparatively studied for all peptides in aqueous solution.</p><!><p>We began studying the overall reaction (Reaction 1) using both QM and MD simulations. We divided the reaction in two parts. Firstly, the intrinsic (or internal) energy of the ET reaction for isolated Tyr or Cys (N and O acetyl capped) residues in vacuum and in implicit water (using PCM approximation) were computed using the B3LYP method, as a measure of the intrinsic radical stabilization capacity of each residue. This reaction would then correspond to an intermolecular reaction analyzed for comparative purposes. The results show that the reaction is only slightly exergonic (−0.1 and −1.6 kcal/mol for vacuum and implicit water respectively) showing that the radical is similarly stabilized in both residues with a slight preference for isolated Cys-S● in water.1</p><p>Afterwards, we computed the overall reaction in vacuum and in implicit water, starting from four random selected snapshots (corresponding to different conformations), two for each TyrO●-(Ala)n-CysSH and two for each TyrOH-(Ala)n-CysS● (n=0, 1, 2, 4) peptide (obtained from explicit water MD simulations) which therefore corresponds to the intramolecular reaction. The data in Table I for the averaged peptides/snapshots show that the reaction is also exergonic (with a mean of ≈ −6.2 and −6.4 kcal/mol in vacuum and in implicit water, respectively), further supporting the higher stabilization of the radical in the Cys residue. No significant dependence on the number of Ala spacers was observed. Moreover, the reactions in vacuum and in implicit water did not show significant differences among them. Interestingly, the reaction was more favorable in the peptides (intramolecular) than for the isolated amino acid residues (intermolecular), probably reflecting that in the peptides the radical located on the Cys thiol group, is stabilized by the larger size of the system.</p><p>In a second step, we computed the change in the solvation Gibbs free energy difference (ΔGSV) between both states (TyrO●-(Ala)n-CysSH andTyrOH-(Ala)n-CysS●), for all peptides using explicit water MD simulations, which provide a better description of the specific water solute interactions as compared to the implicit method. The corresponding value was computed using a thermodynamic integration (TI) scheme (See Methods). Briefly, the method consists in slightly changing the peptide parameters from those corresponding to the TyrO●-(Ala)n-CysSH state, to those for the TyrOH-(Ala)n-CysS● state. In this case the change corresponds to switching the corresponding partial charges of the Tyr and Cys residues, and removing the Cys-SH hydrogen Lennard-Jones parameters, which are slowly created in the position corresponding to that of Tyr-OH. The calculation yielded values of −9 ± 3 kcal/mol for all the peptides, showing that solvation contribution to the reaction is exergonic. No significant differences were observed for the different peptides.</p><!><p>We began our mechanistic analysis by looking at the dynamic structure of theTyrO●-(Ala)n-CysSH and TyrOH-(Ala)n-CysS● (n=0, 1, 2 and 4) peptides in explicit water from a 10 ns long MD simulations of each peptide. The general observation is that all the studied peptides show no appreciable structure, and move forming and breaking intramolecular and peptide-water hydrogen bonds (HB) contacts during the time scale of the simulation. In order to analyze the likeliness of HB formation between the Tyr and Cys residues, we measured the distance probability functions between the Tyr-O● oxygen atom and the Cys thiol hydrogen and that between tyrosine phenol hydrogen and the Cys-S● sulfur atoms in each peptide (the corresponding plots are shown in Supplementary Material, Fig. S1). We also computed the amount of time that a HB interaction is present between the above-described atoms (Table II). An HB was considered established whenever the O-S distance was less than 3.5 Å and the O-H-S angle larger than 140 degrees.</p><p>The analysis of the distance probability plots show that only for the peptide containing one Ala spacer the Tyr phenolic hydrogen (Tyr-HOH) or Cys sulfur hydrogen (Cys-HSH) can be found relatively close (less than 3Å) to the radical atoms although with very low probability (and even less probability for the two Ala spacer). In the YC dipeptide (i.e. without Ala spacers) the hydrogen and radical atoms cannot come closer than 3Å, while for the four Ala peptides they are always further than 5Å. This behavior is further reflected in the data from Table II, showing that conformations in which an internal HB is established are extremely unlikely to be found.</p><p>To get some insight into the kinetics of a possible hydrogen- or time-concerted PCET reaction for the most likely structures, we selected one snapshot for each peptide where Tyr and Cys residues are establishing an HB or are as close as possible, and computed the corresponding reaction energy profile with the scan of the HB donor hydrogen atom difference distance to both the Tyr-O●and Cys-S●atoms as the reaction coordinate. The results for the overall reaction (ΔE) and activation (E#) energies are summarized in Table III.</p><p>The results show that the ΔE for reaction 2 depends considerably on the selected snapshot, but is always smaller that the value obtained for overall computed reaction 1, described previously. This possibly reflects the fact that selected snapshots are biased for structures with HB between donor and acceptor atoms and therefore the hydrogen is similarly stabilized by both residues. Moreover, the computed barriers for Hydrogen/PCET were quite high (> 10 kcal/mol) and no trend is observed for increasing number of alanine spacer residues. The same results were obtained when including one or two waters molecules explicitly in the QM calculation, showing that the barriers are not affected by the presence of the solvent (data not shown). Taken together the fact that almost no structure is found with Tyr-O● and Cys-SH groups (or the Tyr-OH and Cys-S● groups) close or forming an HB, and even for the HB establishing conformations, the relative high barrier for hydrogen or concerted and direct PCET transfer, argue against this mechanism as being operative in the studied peptides.</p><p>A note should be made concerning the computed barrier for hydrogen-transfer or PCET. The barriers computed in the present work do not consider the quantum nature of the transferring atom. Protons and hydrogen atoms could also be transferred by quantum tunneling. Many theoretical studies have been devoted to this issue including calculations in enzymes (28, 46-48). However, analysis of the emblematic cases in comparison to the structures of the peptides show that for the present case donor and acceptor atoms are never found close enough (e.g. < 2 Å) to allow such type of transfer to be significant.</p><!><p>This possibility involves a concerted IET, with a proton transfer through a bridging water molecule, i.e the proton is transferred from the Cys to a water molecule that transfers its proton to Tyr, while ET occurs simultaneously. To analyze this possibility for the above-described peptides, we studied the probability of finding water molecules bridging the Tyr-O and Cys-S interaction in the TyrO●-(Ala)n-CysSH and TyrOH-(Ala)n-CysS● peptides. For this sake we computed the probability that one, two or three water molecules were found to be simultaneously HB Tyr-O to Cys-HS and Tyr-HOH and Cys-S atoms, and considering always the non radical residue as being the HB donor. No structure containing water molecules bridging both reactants was found (not shown), suggesting that water-mediated PCET is highly unlikely.</p><!><p>In this case IET is proposed to occur first, reducing the Tyr-O● radical to phenolate (Tyr-O-), with concomitant Cys oxidation.Afterwards, the proton is released from the resulting cysteine cation radical (Cys-SH●+) and uptaken by the Tyr-O-, either intramolecularly or mediated by the water solvent. To analyze this possibility, we computed the energy associated with the proposed IET. The results obatined from QM calculations for the isolated residues in vacuum and in implicit solvent, yield ΔE values of 135.7 and 50.6 kcal/mol, respectively. This value strongly suggests that IET occurring before Cys deprotonation is extremely unlikely, and therefore this mechanism is unlikely to be relevant in the present case.</p><!><p>In this case the proton and IET steps are proposed to be significantly separated in time, resulting in an acid-base equilibrium three step dependent reaction. First, the Cys side chain acid-base equilibrium allows formation of the negative thiolate moiety (Cys-S−) (Step 1D). In the second step (Step 2D), the negatively charged radical peptide undergoes IET, which in fact corresponds to internal charge redistribution, resulting in Cys-S● and a Tyr-O−. Finally, the Tyr-O− must capture a proton from the solvent in a reaction that corresponds to Tyr acid base equilibrium referred as step 3D. Key processes for the forward and back reactions are the Cys and Tyr acid-base reactions governed by the corresponding pKa values, which are 8.37 and 10.46 (49) for isolated Cys and Tyr residues in water, respectively. In a first approximation we can expect that the corresponding pKa values will remain similar for all peptides, since each residue is completely exposed to the solvent as shown by previously described MD results. Therefore, significant amount of charged Cys can be found for the forward reaction, while once the charge is transferred to the Tyr residue the equilibrium is considerably displaced to the neutral state.</p><p>The kinetics of acid-base phenomena in aqueous solution is determined by the thermodynamics, namely, spontaneous processes are barrierless (50), while those non spontaneous exhibit kinetic rate constants that can be predicted from the Ka values. In this case, using Eyring equation (51)2, the proton transfer rate constant from Cys to water (kCys-) is estimated to be 2.4 × 104 s−1, which is similar or even faster than the estimates for the overall rate reaction in tyrosine-containing peptides k=103-104 s−1 (9, 15, 52) suggesting that ET (Step 2D) being at least partially rate-limiting. On the other hand, tyrosine protonation is spontaneous, and so, it is expected to be diffusion-controlled.</p><p>We now turn our attention to the IET in the charged peptides, corresponding to Step 2D. The reaction corresponds to a change in the negative charge localization from the Cys to the Tyr residue or vice-versa. For two isolated Cys and Tyr peptides in vacuum this results in an energy change of −7.0 kcal/mol, showing a preferred intrinsic stabilization of the negative charge in the Tyr residue. Now, in order to estimate the change in the solvation Gibbs free energy for the charge transfer reaction, the Gibbs free energy change for all the peptides during the charge process in explicit water was computed using TI. In this case the TI protocols consisted in changing the partial charges of the Tyr and Cys atoms, from those corresponding to having a Tyr-O● and Cy-S− to those corresponding to Tyr-O− and Cys-S●. The corresponding free energy change for all the peptides was about 4 kcal/mol positive, showing that solvation favors localization of the radical in the Tyr residue, while preferentially stabilizing the charge in the Cys residue, consistent with the above-described observation for the QM (PCM) results. The higher stabilization of the charged state in the Cys residue is consistent with the lower pKa for Cys compared to that of Tyr. More interestingly, no significant change in the solvation free energy is obtained for the peptides containing increasing number of alanine residues. Finally, combining the results for the intrinsic and solvation effects overall charge transfer from TyrO− to Cys-S●is only slightly endergonic (ca. +3 kcal/mol).</p><!><p>Having analyzed the thermodynamics of Step 2D, we now turn our attention to possible influence on ET kinetics in the different peptides. In this context, and to analyze ET rates in a comparative way for the TyrO●-(Ala)n-CysS− and TyrO−-(Ala)n-CysS● peptides, we first computed the ET coupling matrix (TDA) between Tyr-O and Cys-S located orbitals using the Pathways algorithm (see Methods), using a hundred snapshots taken from the MD simulation of the corresponding peptide. The results are presented in Table IV; although the method is not expected to yield quantitative coupling values, the results are reliable when analyzed as qualitative comparative trends (53).</p><p>As expected if only through bonds ET pathways are considered, the more Ala are inserted between Tyr and Cys residues, the lower predicted coupling values, with relative values of 1, 0.16, 0.046 and 0.002 for peptides containing zero, one, two and four Ala respectively (as determined by the values shown in the 2nd column of Table IV). When looking at the average predicted coupling values (3rd and 5th column) a similar trend is observed but the differences are much smaller. The reason for the smaller differences is due to the fact that, for longer peptides more and more IET pathways are predicted that shortcut between Tyr-O and Cys-S atoms, either through a space jump or through two consecutive jumps involving a bridging water molecule. This trend is so far the only observed difference in computed parameters between different peptides. Moreover, the trend is consistent with the experimentally observed amount of Cys disulfide (Cys-Cys) peptides after tyrosyl radical formation (Table IV); indeed, the reaction yields depend on the nature and extent of radical transfer reactions and are consistent with a relevant role of the IET rate, as determined by the electronic coupling in the present reaction.</p><p>As shown in Equation 1, the ET rate not only depends on the coupling, but also on the reorganization energy, which is defined as the energy needed to distort or move the structure along the reactant surface, to the optimal structure corresponding to that of the products. For the corresponding reaction, possibly most of the reorganization energy will come from the change in peptide solvent interactions as the charge distribution is altered due to the ET process, i.e. how much energy is needed to drive solvent structure to that of the product charge distribution with the distribution corresponding to the reactant state. As a first attempt to estimate the reorganization energy in a comparative manner, we computed the total non bonded energy of the system (intermolecular interactions) for both charge distributions (TyrO●-(Ala)n-CysS− and TyrO--(Ala)n-CysS●) along the TI reaction coordinate (i.e. from ζ=0 to ζ=1) for step 2D which corresponds to the process of changing the charge distribution form one state to the other. The non-bonded energy is expected to capture most of solvation energy, since water molecules have no internal energy in the TIP3P model, and peptide internal energy (bonds and angles) are expected to be approximately the same along the whole reaction. The resulting plots are shown in Supplementary Material (Fig. S2A). The results show that moving forward in the RC (which corresponds to changing ζ from 0 to 1) results in an increase in the TyrO●-(Ala)n-CysS− energy, and a decrease in the TyrO−-(Ala)n-CysS● energy as the solvent structure adopts optimal conformation for solvating the TyrO−-(Ala)n-CysS● charge distribution (corresponding to ζ=1). Interestingly, for all peptides a similar trend for the energy changes are observed and therefore very similar reorganization energy values can be expected.</p><p>To have another estimation of the change in the solvation Gibbs free energy, and therefore the main component of λ (the reorganization energy), and given that main changes in solvation occur for the Tyr-O and Cys-S atoms, we also computed the water charge/radical interaction Gibbs free energy (J(r)) for each TI window as determined by the S/O centered water radial distribution function (g(r)) using the following equation:
(Equation 2)J(r)=−kTln(g(r))
Where J(r) is the Gibbs free energy of the water molecule which interacts with the TyrO and/or CysS atom at distance r, k is the Boltzmann constant and T the temperature. This equation allows the determination of the Gibbs free energy of solvation for the corresponding CysS and TyrO atoms corresponding to each water molecule from the first solvation shell by computing the difference in J(r) between the value in the bulk solvent (set to zero) and the peak in the g(r) function. The resulting plots (see Supplementary Material, Fig. S2B) and the estimated reorganization energy indicate that the differences are much smaller compared to previously computed non-bonded energy. The contribution to the reorganization energy, thus estimated as the end point value for the original charge distribution. (i.e. the value at ζ=1, for the charge distribution of the TyrO●-(Ala)n-CysS− state and vice versa) is predicted to be between 2-4 kcal/mol and similar for all peptides.</p><p>In summary, taking into account the estimation of the coupling and reorganization energies for each peptide, our data indicate that although reorganization energy estimates were similar for all peptides, the coupling and therefore the ET rate significantly decreased with the number of Ala spacers, a fact that is consistent with the experimental data from Zhang et al. (16).</p><!><p>The results presented in the present manuscript can be related to previous studies performed by Prütz et al. where the redox potential of isolated Tyr and Cys amino acids were established at different pH values (10), which show that at pH<8 (i.e. Tyr and Cys are neutral species) the difference in redox potential is ca. zero, implying a minimal change in Gibbs free energy for the intermolecular ET reaction. Interestingly, at pH > 10, where both Cys and Tyr residues are deprotonated the difference in the redox potential for intermolecular ET from Tyr to Cys is increased, and results in a ca. 3.7 kcal/mol for the ΔG of the reaction. Also, more recent studies by Folkes et al. (24) showed that the equilibrium constant for reaction between tyrosyl radicals and glutathione at pH 7.15, is close to one. Our results for isolated neutral aminoacid residues, yielded values which are only slightly exergonic (−1.6 kcal/mol) which, given the approximations involved in the calculations, are in reasonable agreement with the mentioned above experimental values. For the peptides, however, our results (Table I) clearly show that the intramolecular electron transfer process indicated in reaction 1 is exergonic, a fact consistent with the experimental observation by Zhang et al. (16). where the process was actually shown to occur3. Therefore, at least for the neutral peptide there seems to be significant differences in the reaction energetics between the inter- and intra-molecular electron transfer reactions. Also, noteworthy is the fact that our results of the ET reaction in the charged peptides yields a value of +3 kcal/mol (combining the in vacuum intrinsic −7 kcal/mol and the +4 kcal/mol corresponding to solvent contribution), a result that is analogous to the data obtained by Prutz et al. at pH >10 (10).</p><!><p>The present study was partially motivated by previous results of 3-nitrotyrosine and disulfide (Cys-Cys) yields of different Tyr and Cys-containing peptides in water exposed to the oxidizing/nitrating system of myeloperoxidase/H2O2/nitrite (16). In the corresponding work the amount of Cys-Cys product after Tyr-O● formation (which is an indicative of intramolecular radical transfer occurrence corresponding to the overall reaction) showed a clear decrease in the Cys-Cys product with an increasing number of Ala spacers (Table IV); this observation is also consistent with the notion that aliphatic amino acids such as alanine can not act as "stepping stones" or "relay" amino acids during IET processes (7). Taken together, our results strongly suggest that Mechanism D is the most likely. The choice is based on the following reasons. First, mechanism A, B and C are discarded on the following basis. Mechanism A, requires the establishment of a strong HB between Tyr and Cys residues, a conformation which is present less than 1% of the time, as determined by the explicit water MD simulations. Moreover, even for these structures the barrier for HAT or PCET are larger than 10 kcal/mol. Mechanism B is discarded, since the required water bridged structure/conformation is never obtained. Finally, for mechanism C the ab-initio calculations yield a possible barrier value of more than 50 kcal/mol, clearly showing that the mechanism C is extremely unlikely. Second, and more important, for mechanisms A, B and C no difference in any of the computed parameters is obtained for the different Ala-containing peptides. The only significant difference among the different peptides was obtained for the electronic coupling term that is expected to control the ET rate in mechanism D, since also both estimations of the reorganization energy yielded no significant differences among the peptides. The predicted relative ET rates according to estimated couplings (presented in Table IV), are consistent with the observed experimental trend in the amount of inter-peptide disulfide formation. The observed consistency makes a strong point for Mechanism D as the responsible for the radical transfer mechanism in the system. The overall proposed mechanism, which involves three steps (Step 1, 2 and 3D), is shown in Figure 3.</p><p>It is important to note, that under the proposed mechanism, the effective overall observed reaction rate, as experimentally determined, will result from a combination of the ET rate and pKa equilibrium. For example, at pH 7.4 (16), there is already a significant amount of deprotonated Cys (ca. 10 %), which undergoes oxidation via reactions step 2D. For this subpopulation the rate is directly the ET rate, and final reprotonation of TyrO- is expected to be very fast as mentioned previously. For the protonated Cys species the estimated rates for Step 1D (based on pKa value) are similar to the electron migration rates in the peptide unit as reported by (9, 15, 52). On the other hand, the rate of this step is expected to be similar in the studied peptides of different length. Conversely, the trends in the computed electronic coupling terms, which are related to the electron transfer rate constants through Marcus equation (Step 2D), correlate very well with the experimental trends (Table IV), indicating that this a key rate-controlling step in the overall radical transfer process.</p><p>Our QM and MD results show that although intrinsically the Cys to Tyr charge transfer is exergonic, the solvent tends to localize the charge on the Cys residue. Our best estimation, combining QM value for Cys-S− to Tyr-O● electron transfer in vacuo (−7 kcal/mol) and the +4 kcal/mol corresponding to the change in solvation Gibbs free energy determined from the TI calculation, yield a value of ca. −3 kcal/molfor the charge transfer reaction in all of the studied peptides. Given the approximations, the relative small value obtained suggests that once deprotonated radical states localized in either Tyr or Cys residues in each peptide maybe significantly populated (i.e. the equilibrium constant for IET, KIET, must be not very far from unity). Therefore, the outcome of the oxidation process yielding Tyr nitration, Cys nitrosation or any of the other routes shown in Figure 1, will depend on the presence of the secondary reactants such as ●NO2, ●NO and oxygen.</p><p>It should be noted however, that in proteins the local environment of each residue may stabilize/destabilize either the radical or charged state of the residue therefore, favoring a specific modification. The fact that radical transfer mechanism occurs through a deprotonation/protonation coupled with internal ET mechanism, may explain why Cys are able to inhibit Tyr nitration but neighboring Met are not, as experimentally observed for model peptides (16). Also important is the fact that for the overall radical transfer reaction from the Tyr to Cys residue (Reaction 1), our results show a gained energy both in the gas phase (QM) and in water (MD), suggesting that the overall intramolecular reaction in small peptides is thermodynamically favored by about −15 kcal/mol, as estimated by adding the intrinsic energy difference from Table I and the change in the solvation Gibbs free energy estimated with the TI scheme. This result is consistent with the experimental observation that nearby Cys residues are able to reduce Tyr-O● yielding the more stable radical Cys-S● resulting in the formation of Cys-Cys bridges or Cys S-nitrosation (16).</p><p>Finally, our results suggest that most likely mechanism involves acid/base equilibrium and IET in the charged peptides. On the other hand, direct hydrogen, direct concerted PCET or even water-mediated transfer are very unlikely to occur, since during the simulation, hydrogen (or proton) donor and acceptor atoms never are close enough for establishing proper HB interactions. Moreover, even for the best possible structures the instantaneous reaction, which usually corresponds to a PCET, has a quite high barrier. Nevertheless, the lack of intramolecular HB could be just a particular property of the present studied model peptides. In proteins, the local structure may impose direct or another residue-mediated HB between Tyr and Cys residues, therefore making possible a direct concerted PCET mechanism (20, 39, 54-57).</p><!><p>In the present work we have analyzed several mechanistic possibilities at the atomic level for a radical transfer reaction in Tyr/Cys-containing peptides in aqueous solution. Our results show that tyrosine radical (Tyr-O●) to cysteine transfer is most likely to be mediated by an acid/base equilibrium that involves deprotonation of cysteine residue to form thiolate, followed by a fast (e.g. k≅103-104 s−1 in tyrosine-containing peptides (9, 15, 52)), yet rate limiting, internal electron transfer (i.e. charge redistribution) resulting in cysteine radical and tyrosine phenolate. Finally, proton uptake by the Tyr residue completes the reaction. Therefore, the reaction can be described as a sequential, acid base equilibrium-dependent and solvent-mediated PCET reaction (Figure 3); within this general mechanism, electrons could be physically transferred via alternative pathways depending on the nature of the peptide and/or polypeptide chain sequence and structure and the characteristics of the milieu (Figure 4). While in short peptides the through-bond path (Type A) predominates, as the peptide chain lengthens, the jumping pathways of type B and C shown in Figure 4 become more likely. For one alanine spacer through space jumping paths (Type B) correspond to ca. 10%, while water-mediated paths are rare. For the two and four alanine spacers jumping paths are between 20 to 35% and equally distributed between types B and C. The resulting Cys-S● can evolve to secondary end products, which may vary depending on reaction conditions (16) (as shown in Figure 1), including cysteine disulfide (Table IV). The overall mechanism fully explains the dependency of tyrosine nitration and cysteine disulfide yields as a function of the number of Ala spacers observed experimentally previously (16). Importantly, while the capacity of ET will be highly influenced by the pKa of the corresponding amino acids, the intramolecular ET process will be in competition with direct bimolecular reaction of the primary radicals (e.g. Cys●) with reactants such as oxygen, nitric oxide (●NO), nitrogen dioxide (●NO2), and other radical species or even spin traps (see Figure 1). The pKa of Cys and Tyr will be influenced both by neighboring amino acids as well as by the degree of hydrophobicity of where they are located. Indeed, hydrophobicity will increase the pKa value (58) and therefore possibly hinder the ET process. Our study has focused on model peptides with an in silico approach and taking into consideration previous experimental data on Tyr-Cys-containing peptides (16) and protein oxidation (17, 18). These findings provide new elements to mechanistically and kinetically explain more probable oxidative modifications in proteins that contain Tyr-Cys sequences and may assist in the understanding of the cytoprotective actions of tyrosine- and/or cysteine-containing peptides during oxidative stress conditions in vitro and in vivo (59, 60).</p>