There are three types of Osmosis solutions&colon; the isotonic solution, hypotonic solution, and hypertonic solution. While they may start out hypertonic they become hypotonic solutions as the sugar is metabolised. A hypotonic solution has a lower concentration of solutes than another solution. This hypotonic filtrate is passed to the distal convoluted tubule in the renal cortex. Spheroplasts are osmotically fragile, and will lyse if transferred to a hypotonic solution. In freshwater environments, the concentration of solutes is hypotonic, higher inside than outside the cell. True hyponatremia, also known as hypotonic hyponatremia, is the most common type. The term "hypotonic hyponatremia" is also sometimes used. The cytosol is conversely categorized as hypotonic, opposite of the outer solution. In hypotonic conditions (freshwater), the cell absorbs water by osmosis. These muscles are hypotonic, atrophic and have no spontaneous contraction. When in a hypotonic solution, water flows into the membrane and increases the cell's volume. Fresh water creates a hypotonic environment for aquatic organisms. Cells are then trypsinized and resuspended in a hypotonic buffer. Plasmolysis can be reversed if the cell is placed in a hypotonic solution.