Pandoravirus enters amoebas through phagocytic vacuoles, then fuses with the membrane vacuole of the amoeba. In these cells the cytoplasm forms only a peripheral layer with a large central vacuole. Strands of cytoplasm often run through the vacuole. Most plants store chemicals in the vacuole that react with chemicals in the cytosol. "Chara corallina" exhibits cyclic cytoplasmic flow around a large central vacuole. It also showed that Uth1p protein is necessary to move mitochondria to the vacuole. Farnesylation allows AnkB to get anchored into the cytoplasmic side of the vacuole. A phagosome is a vacuole formed around a particle absorbed by phagocytosis. Immediately after the contractile vacuole (CV) expels water, its membrane crumples. This sequestration mechanism is facilitated by proteins on the vacuole membrane. The cyst wall is formed by the parasitophorous vacuole membrane. The contractile vacuole, as its name suggests, expels water out of the cell by contracting. The nematocyst forms through a multi-step assembly process from a giant post-Golgi vacuole. Blister cells appear as red blood cells containing a peripherally located vacuole. Infected cells usually possess a large vacuole.