Before ion-exchange chromatography can be initiated, it must be equilibrated. Effusion from an equilibrated container into outside vacuum can be calculated based on kinetic theory. The negative temperature ensembles equilibrated and showed long lifetimes in an anti-trapping harmonic potential. Temperature is, by definition, proportional to the average internal energy of an equilibrated neighborhood. If menthone and isomenthone are equilibrated at room temperature, the isomenthone content will reach 29%. The elimination of de-motivation results in a more equilibrated theory.