Conventional PCR requires primers complementary to the termini of the target DNA. Heuston and Connolly stations are the two main railway termini in Dublin. Piraeus station refers to the two railway termini located next to the seaport. The A and B lines intersect at Roma Termini station. Both its northern and southern termini are interchanges with I-135. Long-distance intercity services are provided at some London rail termini. This method will be very useful in the case of polypeptides and protein-blocked N termini. Altrincham Interchange is one of the Metrolink's termini. The third rail extended throughout most South London lines out of all its London termini. Transfers are available with Line A and other rail services at Termini Station. This same practice applies to all the London mainline rail termini, except London Bridge. There are 298 bus termini in Hong Kong. One train leaves from these two termini each. This required trains to alternate between the two southern termini. There are two main termini for bus services.