These fragments are then ligated together to generate chimeric genes. These RNA nucleotides are ligated, resulting in a strand of messenger RNA. Finally, the processed coding ends are ligated together by DNA ligase IV. Subsequently, interacting loci are quantified by amplifying ligated junctions by PCR methods. These monomers are then ligated to form circular RNA. Short adaptors were then ligated onto the ends of the fragments. These resulting fragments are then ligated together to form full length genes. Barcode adapters are then ligated to sticky ends and PCR amplification is performed. Okazaki fragments that are not ligated could cause double-strand-breaks, which cleaves the DNA. More rarely the maxillary or a branch of the external carotid artery can be ligated. It can be ligated surgically to control such nosebleeds. In addition, it only produces small amount of fractionated DNA ligated products. Strands that fail to have adapters ligated are washed away. Here, a 100bp duplex DNA piece is partially ligated. The procedure stops bleeding from small vessels (larger vessels being ligated).