Aromatic rings are acetylated by acetic anhydride. Acetalated dextran is structurally different from acetylated dextran. Finally, the amine is acetylated with acetic anhydride. Genes get turned on if this mark is acetylated and silences them if methylated. The oxime is then acetylated and hydrolyzed to yield the natural product hemiacetal. PCAF may be autoacetylated (acetylated by itself) or by p300. Acetylcholine is a choline molecule that has been acetylated at the oxygen atom. This acetylated state requires hydrolysis to regenerate itself. The acetylated, hydroxylated, and conjugated forms have little antibacterial activity. This secondary amine is acetylated with chloroacetylchloride. When the acetylized composition of the copolymer is over 50% acetylated it is chitin. However, to be functional, ATM protein kinase must be acetylated by the KAT5 protein. This selectivity was reversed when the allylic alcohol was acetylated. In the final step, the cystine residue in the conjugate is acetylated. In bacteria, 90% of proteins involved in central metabolism of "Salmonella enterica" are acetylated.