Some tumor cells overexpress specific glycolytic enzymes which result in higher rates of glycolysis. The aldolase used in gluconeogenesis and glycolysis is a cytoplasmic protein. Pyruvate oxidation is the step that connects glycolysis and the Krebs cycle. Gluconeogenesis is the reverse process of glycolysis. Before fermentation, a glucose molecule breaks down into two pyruvate molecules (Glycolysis). Pyruvate kinase is the enzyme involved in the last step of glycolysis. There are two steps in the pyruvate kinase reaction in glycolysis. These metabolic processes are known as glycogenolysis, glycolysis and lipolysis. Usually this is pyruvate formed from sugar through glycolysis. The pyruvate generated as an end-product of glycolysis is a substrate for the Krebs Cycle. TIN2 can localize to mitochondria where it promotes glycolysis. Restoring NAD levels is essential to the continuation of anaerobic energy metabolism through glycolysis. The biosynthesis of coumarin in plants is via hydroxylation, glycolysis, and cyclization of cinnamic acid. Uridine plays a role in the glycolysis pathway of galactose. It can also hydrolyze lactose into galactose and glucose which will proceed into glycolysis.