Denaturing alcohol does not chemically alter the ethanol molecule unlike the denaturation process in biochemistry. Dry heat destroys microorganisms by causing denaturation of proteins. Moist heat causes the destruction of microorganisms by denaturation of macromolecules, primarily proteins. This method includes three steps; denaturation, annealing, extension. Biochemical studies indicate AgRP to be very stable to thermal denaturation and acid degradation. DNA Helicase, an enzyme that unwinds DNA, is used in place of thermal denaturation. It has three main steps&colon; denaturation, annealing, and extension. The denaturation of hemoglobin overwhelms the capacity of the G6PD metabolic pathway. The process can induce both protein denaturation and starch gelatinization under some conditions. The hydrophobic effect depends on the temperature, which leads to "cold denaturation" of proteins. Severe changes in pH will lead to denaturation of the enzyme. Ozone sterilization is currently being studied as a potential method for prion denaturation and deactivation. The denaturation of these proteins happens in two steps. This model describes the denaturation of DNA strands as a function of temperature. Form IV (green curve) is the product of alkali denaturation of Form I.