Then, an interphase or metaphase chromosome preparation is produced. During interphase, PLK1 localizes to centrosomes. The homogeneous pattern is seen when the condensed chromosomes and interphase chromatin stain. This mirrors the major phases of cell cycle&colon; interphase and mitosis. Beginning after interphase, DNA has already been replicated when the cell enters prophase. Mitosis and cytokinesis, however, are separate from interphase. Studies have shown that during mitosis 15% of all interphase FoxA1 binding sites were bound. Microtubules involved in the interphase scaffolding break down as the replicated centrosomes separate. During all three parts of interphase, the cell grows by producing proteins and cytoplasmic organelles. There is evidence that these regions are important to the structural formation of interphase chromosome. PIKA domains, or polymorphic interphase karyosomal associations, were first described in microscopy studies in 1991. The progression of interphase is the result of the increased amount of cyclin. This austenisation of white iron occurs in primary cementite at the interphase boundary with ferrite. DNA replication occurs during the S-stage of interphase. After cell division, each of the daughter cells begin the interphase of a new cycle.