Recombinases are capable of pairing oligonucleotide primers with homologous sequence in duplex DNA. The breech mechanism has a primer magazine holding 18 primers. Reagents required for MDA reactions include&colon; random primers and DNA polymerase from bacteriophage phi29. Ligated fragments are detected using PCR with known primers. The cDNA is then amplified using two target specific PCR primers. PCR primers and probes including SYBR Green primers, TaqMan Probes, Molecular Beacons. This is particularly useful when testing suitable annealing temperatures for PCR primers. Many different companies have developed diagnostics tests based on ARMS PCR primers. Next, primers and modified nucleotides enter the chip. These were often reacted with styrene to make styrenated epoxy esters, used as primers. However, the efficiency of amplification is often variable among primers and templates. The RNA is then reverse transcribed using barcoded primers. Misfires and hang fires became common as the remaining priming compound sputtered in old primers. This includes waterborne epoxy resin system for making zinc-rich primers. Most centerfire brass cases used worldwide for sporting ammunition use Boxer primers.