Ascospores are stained with Kinyoun stain and ascospore stain. There has been no correlation between light, temperature, and ascospore dispersals in field studies. Typically, one side of the ascospore is flattened with 3 longitudinal rims. All kinds of mutants are easily induced and readily obtainable with particular ascospore color mutants. Despite this, researchers have not been able to associate any specific ascospore with "H. hyalinus". Two haploid nuclei originating from the same meiosis are packaged into one ascospore. Ideal ascospore dispersal occurs after nightly rainfall and dew periods . Ascospore release is synchronized with the foliar development of the elms. "P. pastoris" can undergo both asexual reproduction and sexual reproduction, by budding and ascospore. Ascospore formation is accelerated by sequential wetting and drying of the leaf litter. Its ascospore morphology was identical to that of "Aspergillus thermomutatus". However, very wet conditions inhibit ascospore development due to leaf decomposition and competition from saprophytes. Under repeated episodes of wetting and drying for up to week, ascospore release can occur.