Membrane lipid biosynthesis occurs in the endoplasmic reticulum membrane. This protein localizes to the endoplasmic reticulum. RNA translation occurs inside the endoplasmic reticulum. The majority of proteins synthesized in the rough endoplasmic reticulum undergo glycosylation. The outer nuclear membrane is continuous with the endoplasmic reticulum membrane. Redox dysregulation leads to increases in nitrosative stress in the endoplasmic reticulum. De novo synthesis of ceramide occurs in the endoplasmic reticulum. These structures consist of rough endoplasmic reticulum and associated ribosomal RNA. Assembly of the icosahedral capsid occurs on modified membranes from the endoplasmic reticulum. Club cells accomplish this with cytochrome P450 enzymes found in their smooth endoplasmic reticulum. These enzymes belong to cytochrome P450 group present in the smooth endoplasmic reticulum. PTP1B is localized to the cytoplasmic face of the endoplasmic reticulum. Soluble inositol-(1,4,5)-trisphosphate triggers calcium to be released from the endoplasmic reticulum. Ribosomes are often associated with the intracellular membranes that make up the rough endoplasmic reticulum. Ribosomes can be found floating within the cytoplasm or attached to the endoplasmic reticulum.