The Arp2/3 complex also regulates the intracellular motility of endosomes, lysosomes, pinocytic vesicles, and mitochondria. These vacuoles include lysosomes filled with acid phosphatase. It is primarily present at the Golgi complex, endosomes, lysosomes, and secretory granules. Enzymes of the lysosomes are synthesised in the rough endoplasmic reticulum. Early endosomes then "mature" into late endosomes before fusing with lysosomes. The osteoclasts are multi-nucleated cells that contain numerous mitochondria and lysosomes. This occurs by diffusion of ambroxol into lysosomes and lysosomal pH neutralization. AP3 is responsible for protein trafficking to lysosomes and other related organelles. Weak bases with lipophilic properties accumulate in acidic intracellular compartments like lysosomes. LRP2/megalin functions to mediate endocytosis of ligands leading to degradation in lysosomes or transcytosis. Macrophages possess a repertoire of antimicrobial molecules packaged within their granules and lysosomes. Their cytoplasm is eosinophilic and contains variable amounts of lysosomes. The enzymes are packed into vesicles for further transport to established lysosomes. Finally, the endosomes lose RAB5A and acquire RAB7A, making them competent for fusion with lysosomes. The bound TLF is endocytosed via coated vesicles and then trafficked to the parasite lysosomes.