Juana is artificially inseminated with Mauricio's semen. Three female chimpanzees were inseminated with human sperm, he failed to create a pregnancy. Beau explains his wife wanted a biological child and was artificially inseminated to become pregnant. Meanwhile, Isadora wants to be artificially inseminated. Amy and Kevin divorced, and she was artificially inseminated by a Dr. Brian Neeves. Orkid has been artificially inseminated many times, but has never gotten pregnant. John reveals that he arranged for Abhinaya to be inseminated with his own sperm. The gestation period occurs once the female has been inseminated from the male successfully. Colonies are annual with only inseminated queens overwintering. The potential queen may or may not be inseminated or have developed ovaries. The colony is founded by a single overwintered, inseminated queen. The males eventually die, and the inseminated queen begins diapause. Inseminated and uninseminated queens enter hibernation following a cycle. Several hens can be inseminated from each collection, so fewer toms are needed. Inseminated queens start to search for nesting sites in late April.