The label oligonucleotide and the branched DNA then detects the immobilized target nucleic acid. The mechanism behind exon skipping is a mutation specific antisense oligonucleotide (AON). Recombinases are capable of pairing oligonucleotide primers with homologous sequence in duplex DNA. Targets can be reliably imaged through the application of multiple short singly labeled oligonucleotide probes. A target-specific probe, composed of 20 oligonucleotide pairs, hybridizes to the target RNA(s). This antibody is also attached to an oligonucleotide primer. The initial pool can also be derived from amplification of a single oligonucleotide strand. SNP detection through molecular beacons makes use of a specifically engineered single-stranded oligonucleotide probe. The OB-fold is a small structural motif originally named for its oligonucleotide/oligosaccharide binding properties. The digested vector is cut with a restriction enzyme that complements the oligonucleotide insert overhangs. An oligonucleotide containing the desired mutation is used for primer extension. A limited version of the RFLP method that used oligonucleotide probes was reported in 1985. Its primary use has been in labeling DNA and RNA in automated oligonucleotide synthesizers. This site saturation mutagenic technique begins with one mutagenic oligonucleotide and one universal flanking primer. The second step then involves connecting these oligonucleotide fragments using various DNA assembly methods.