In synaptic transmission in neuronal cells, V-ATPase acidifies synaptic vesicles. It acidifies the feed thus preventing microbe growth and increasing shelf life. Smelters also release gaseous sulfur dioxide, contributing to acid rain, which acidifies soil and water. The common presence of peat bogs on Ireland acidifies stormwater. The second part of the Winkler test reduces (acidifies) the solution. Translocation occurs through a series of events which begin in the endosome as it acidifies. This reaction acidifies the environment and the Fe will be formed is rather stable.