If the diluent is unknown, equilibrium supernatant is readily obtained by centrifugation. A serum is the clear supernatant after the precipitation of the cloud through centrifugation. They can easily be transfected either with viral supernatant or physical methods. The remaining solution (supernatant) may be discarded with a pipette.. After 5 minutes, carefully decant the supernatant solution and determine the fluoride. The albumin remains in the supernatant fraction during the solid/liquid extraction under these conditions. ISG15 is secreted from the cell and can be detected in supernatant or blood plasma. The filtration method facilitates purification of toxin B from the supernatant of "C. difficile". Mitotic cells can therefore be completely detached by gently shaking and collected from the supernatant. Then the supernatant in each well can be checked for the desired antibody. The toxin concentration of the supernatant is proportional to the organism cell count. The lentiviral particle-containing supernatant is harvested, concentrated and subsequently used to infect the target cells. Centrifugation of protein solution, for example, allows elimination of impurities into the supernatant. The supernatant containing contaminants can be carefully removed so as not to disturb the beads. The formation of the cellulose pellicle occurs on the upper surface of the supernatant film.