Its sap contains the deadly cardiotoxic glycoside "ouabain". The glycosylation of ouabagenin with rhamnose produced ouabain. It is the aglycone of k-strophanthin, an analogue of ouabain. The structure of E was confirmed by comparison against the degradation product of ouabain. The figure below shows the key steps in the synthesis of ouabain. Post asked whether the enzyme was inhibited by ouabain. E contained all the required functionalities and stereochemistry needed to produce ouabain. Na/K-ATPase by binding to the same receptor sites as the digitalis glycosides such as ouabain. It is however, just like its derivate oleandrigenin, a more potent glycoside than ouabain. Ataxia is observed for lower ouabain concentrations, dystonia is observed at higher ouabain concentrations. However, a high dose of ouabain can lead to ventricular tachycardia. The total synthesis of ouabain was achieved in 2008 by Deslongchamps laboratory in Canada. Ouabain infusion decreases ventricular escape time and increases ventricular escape rhythm. This plant contains ouabain, which is a cardiac glycoside, oleander, and milkweeds. Ouabain is eliminated by renal excretion, largely unchanged.