Amplexus occurs in all four species of horseshoe crab. This culminated in the Creek defeat at the Battle of Horseshoe Bend. Natural breeding of horseshoe crabs in captivity has proven to be difficult. The harvesting of horseshoe crab blood in the pharmaceutical industry is in decline. A horseshoe magnet is a magnet made in the shape of a horseshoe. Hamilton is in the geographic centre of the Golden Horseshoe. Rhinolophidae is a family of bats commonly known as horseshoe bats. The Greater Toronto Area anchors a much larger urban agglomeration known as the Golden Horseshoe. The enigmatic Chasmataspidids are also thought to be closely related to the horseshoe crabs. It is the southern end of the Golden Horseshoe, the largest megalopolis in Canada. The mating systems of horseshoe bats are poorly understood. They are both in the eastern part of the Golden Horseshoe region. The biogeography of horseshoe bats is poorly understood. The census metropolitan areas listed below are within the Greater Golden Horseshoe. Universities' athletic teams in the Golden Horseshoe belong to U Sports.