I then eluted into an alkaline solution. Finally, the pure product is eluted with a different buffer. The rest of the RNAs are eluted out. The enzyme can then be eluted out by adding buffer with higher salt concentrations. The hydrazones are then eluted and analyzed by HPLC using a UV detector. Typically, the analytes are eluted in an order determined by their pKa values. This will cause desired proteins to be eluted out of the column. Firstly, CK- MM was eluted, then CK-MB, followed by CK-BB. The target protein can be eluted by changing the pH or the salinity. Unhybridized fragments are washed away and the desired fragments are eluted. The DNA can be eluted from the silver stain for further analysis through PCR amplification. Bound proteins are eluted out by utilizing a gradient of linearly increasing salt concentration. Amino acids are eluted when the pH reaches their respective isoelectric points. The desired molecules are eluted with specific substances after washing the non-interacting molecules away. Non-specific hybrids are removed by washing and the target DNA is eluted.