The germ layers are referred to as the ectoderm, mesoderm and endoderm. Dorsoventral patterning is mediated by Wnt7a signals in the overlying ectoderm not the mesoderm. BMP4 antagonizes organizer tissue and is expressed in early development in ectoderm and mesoderm tissue. Each structure originates from a different germ layer&colon; the ectoderm, endoderm and mesenchyme. The amnionic ectoderm and extraembryonic mesoderm also originate from the epiblast. Limb bud is undifferentiated mesenchyme enclosed by an ectoderm covering. During this time ectoderm and mesoderm of the frontonasal process produce the midline septum. These three "germ layers" are known as the ectoderm, mesoderm, and endoderm. The outer layer is known technically as the ectoderm, the inner layer as the endoderm. The eardrum is made up of three layers (ectoderm, endoderm and connective tissue). They are diploblastic, having only two main germ layers, ectoderm and endoderm. In diploblastic animals only the ectoderm and the endoderm are present. The cellular tissues that ultimately become teeth originate from the embryonic germ layer, the ectoderm. The embryo is covered on the dorsal surface by a layer of cells called ectoderm. Each tracheal tube develops as an invagination of the ectoderm during embryonic development.