These secondary interactions induce bending of the DNA and helical unwinding. Wrap the rope at that point to prevent it unwinding further. Quinolones exert their antibacterial effect by preventing bacterial DNA from unwinding and duplicating. A transcription bubble occurs as a result of the double stranded DNA unwinding. DNA helicases are responsible for unwinding the double-stranded DNA during chromosome replication. As part of this complex, it facilitates nucleotide excision repair by unwinding DNA. Rescission is the unwinding of a transaction. This allows the normal unwinding of DNA to occur during replication or transcription. It is a replication initiation factor which promotes the unwinding of DNA at "oriC". UBE3A. Topoisomerases are enzymes that regulate the unwinding of DNA. As cells have evolved mechanisms for resolving (i.e., unwinding) quadruplexes that form. This mode of unwinding is used by the DEAD/DEAH box helicases. Dicer processing of the pre-miRNA is thought to be coupled with unwinding of the duplex. I, an enzyme that allows DNA unwinding. It is the generation of negative supercoiling that causes the unwinding.