When grown on nutrient agar, they form unpigmented colonies. Colony hybridization begins with culturing sparsely populated bacterial colonies on a nutrient agar plate. Nutrient agar is also similar to TSA. Nutrient agar plates were used to cultivate the samples. Such a culture can be kept forever by successive transplantations onto fresh nutrient agar. For definitive diagnosis, the pathogen should then be cultured on reduced nutrient agar. The nutrient agar has a lot of ingredients with unknown amounts of nutrients in them. This NGM medium contains nutrient agar (NA) and glycerol medium supplemented with MnCl &colon;4HO. It is also able to grow on TSA, Nutrient Agar, Mac Conkey or CASA Agar.