MAD1 localizes to unattached kinetochores while binding strongly to MAD2. Birds encountered singly are often unattached males, their territories generally in less suitable areas. When kinetochores are unattached to spindles, mitotic checkpoint complexes (MCC) and inhibit APC. Even a single unattached kinetochore can maintain the spindle checkpoint. The tip and the margins are unattached and the central portion attached. Periodically, some men continue to compete individually in an unattached status. The Reversed Queen of Pentacles can become unattached from reality, leaving her completely self-centered. And if real property is severed from the land (rendered unattached) it becomes personal property. Roy joined a group of unattached male penguins. These unattached persons had to be fed and housed. No development takes place as long as the embryo remains unattached to the uterine lining. The pupa is unattached in an earth cell. It does not have to be separate and unattached. Photographs have been taken in which unattached streamers are clearly visible. Ascetics, by tradition, led a nomadic and unattached lifestyle.