Under regulation by gonadotropic hormones, ovarian follicles mature and estrogen secretions exert their biggest influence. These gonadotropic hormones lead to sexual maturation and gametogenesis. Specifically, the study focused on secretion in porcine theca and granulosa cells, controlling gonadotropic stimulation. These gonadotropic hormones play a role in stimulating estrogen release from the ovaries. They include thyroid stimulating hormone (TSH), gonadotropic hormones (FSH, LH), adrenocorticotropic hormone, and prolactin.