The distribution of products from conjugative hydrocyanation depends both on substrate structure and reaction conditions. Plasmids can be broadly classified into conjugative plasmids and non-conjugative plasmids. The protein has a homologue that is encoded by many large, conjugative plasmids. T4SS is encoded on Gram-negative conjugative elements in bacteria. Both pathogens rely on a conjugative virulence plasmid to cause disease. Conjugative hydrocyanation has been heavily used for steroid synthesis. Integrons were initially discovered on conjugative plasmids through their role in antibiotic resistance. The "H. defensa" genome holds a 2,110,331-bp circular chromosome and a 59,034-bp conjugative plasmid. Conjugative replication may require a second nick before successful transfer can occur. Esters, nitriles and other carbonyl derivatives also undergo conjugative hydrocyanation. The Ti and Ri plasmids are themselves conjugative. Conjugative suffixes and auxiliary verbs are attached to the stem forms of the affixee. Conjugative plasmids contain a set of transfer genes which promote sexual conjugation between different cells. They can parasitize a conjugative plasmid, transferring at high frequency only in its presence. Beyond a requirement for base-stable functionality, a wide variety of carbonyl substrates undergo conjugative hydrocyanation.