ILRs are found on the cellular membrane of plant protoplasts. Instead, regenerating moss protoplasts behave like germinating moss spores. The dispersion of the ILRs on these protoplasts can vary from species to species. The DNA construct is then incubated with moss protoplasts in the presence of polyethylene glycol. After dispersal, these four-nucleate spores germinate and naked protoplasts emerge. His protista were divided into moneres, protoplasts, flagellates, diatoms, myxomycetes, myxocystodes, rhizopods, and sponges. It also produces a chitinase A able to produce protoplasts from "Schizophyllum commune" cultured mycelia. Using protoplasts of "A. thaliana" protein-protein interactions can be studied in plants. In this case protoplasts are fused, usually in an electric field. Gram-positive bacteria are called protoplasts when they lose their cell walls. Protoplasts differ from spheroplasts in that their cell wall has been completely removed. Protoplasts may also be used for plant breeding, using a technique called protoplast fusion.