After antibody binding, the membrane is incubated with a chemiluminescent substrate and imaged. The antibody solution is incubated with the membrane for anywhere from 30 minutes to overnight. The resulting viruses are then incubated in chicken eggs and chick kidney cells. Blood serum is incubated in the wells of the plate and is washed out. In March 1990, eggs on Mir were successfully incubated and hatched. The female usually lays two eggs, which are incubated by both sexes. The eggs are incubated for at least 28 days. The egg is incubated around 30 days. Incubated by both parents, the eggs hatch after . Two eggs are laid but only one is usually incubated. In extreme cases, infected individuals may be incubated and receive oxygen therapy. The eggs are incubated by the females by day and by the males by night. The sample is first incubated for 30 min. Eggs are incubated by the female for 12 or 13 days. The eggs are incubated by both parents for .