Birds of a flock will preen each other, ruffling their head feathers in invitation. Ruffling of feathers indicates fear and the will to escape. He noted that the character would arrive "ruffling a few feathers". It is possible that this display originates from a feather ruffling reflex to alarm. Membrane ruffling creates membrane pockets which capture and engulf the bacteria. This led to his habit of ruffling his hair or constantly moving about. She then becomes aware of another sound, the sound of a silk dress ruffling. Ruffling under the silk-like muscles in a nervous horse. N17rac and V12rac1 prevented growth factor-induced membrane ruffling. Rac proteins controls the activities of membrane ruffling and NADPH-oxidase neutrophil. Membrane ruffling is a characteristic feature of many actively migrating cells. The GTP-binding protein Rac is the regulator of this membrane ruffling. These ipa proteins induce "membrane ruffling" by the host cell. Lettuce big-vein disease causes leaf distortion and ruffling in affected lettuce plants. ADF/cofilin is found in ruffling membranes and at the leading edge of mobile cells.