Ross discovered in 1897 that malarial parasites lived in certain mosquitoes. The shallow waters meant that malarial mosquitoes prospered. It was Golgi who helped him prove that malarial parasite was a microscopic protozoan. The patient was suffering from malarial parasites. This new method differentiated blood cells and demonstrated the nuclei of malarial parasites. Children living in areas prone for malarial infections are also at risk of developing anemia. Those with the benign sickle trait possess a resistance to malarial infection. People with sickle cell disease living in malarial countries should receive lifelong medication for prevention. This inhibits the malarial dihydrofolate reductase enzyme. Sixtus V died on 27 August 1590 from malarial fever. Inner Terai valleys historically were agriculturally productive but extremely malarial. Glucose-6-phosphate dehydrogenase human polymorphism is also implicated in malarial resistance. Mutations in the malarial gene for dihydrofolate reductase may reduce its effectiveness. The syndrome is sometimes called "malarial hepatitis". This happened in areas of predominant malarial cases.