All three plasmids are digested with appropriate restriction enzyme and then allowed to ligate. It can also ligate blunt-ended DNA with much greater efficiency than "E. coli" DNA ligase. He is regarded as the first surgeon to successfully ligate the subclavian artery. FANA XNAzymes also showed the ability to ligate DNA, RNA and XNA substrates. Prostaglandins ligate a sub-family of cell surface seven-transmembrane receptors, G-protein-coupled receptors. The "Xbal" and "SpeI" sites will also ligate as the digestion produces compatible ends. The "EcoRI" sites will ligate since they are complementary to each other.