People found to have reactive tuberculin skin tests should be screened for active tuberculosis. Both of these tests use the tuberculin derivative PPD (purified protein derivative). Diagnosis of latent TB relies on the tuberculin skin test (TST) or blood tests. This assessment may include a chest radiograph and tuberculin skin test. A reactive tuberculin skin test is a contraindication to BCG. In 1890 Koch developed tuberculin, a purified protein derivative of the bacteria. Except in neonates, a tuberculin skin test should always be done before administering BCG. By the 1940s, Seibert's PPD was the international standard for tuberculin tests. TST (tuberculin skin test) positive is measured by size of induration. These were also evaluated at the institute, as was tuberculin and later on various vaccines. The tuberculin reaction was first described by Robert Koch in 1890. The tuberculin skin test fails due to the high numbers of false negatives. The seals have paid for millions of Canadians to have chest X-ray or tuberculin tests. The Heaf tuberculin skin test was used in the United Kingdom, but discontinued in 2005. Her first publication on the purification of tuberculin appeared in 1934.