Minipreps are used in the process of molecular cloning to analyze bacterial clones. For Sanger sequencing, either cloning procedures or PCR are required prior to sequencing. In 2001, Capito voted for a bill to ban the cloning of human embryos. The possibility of human cloning has raised controversies. They warned lawmakers against failing to regulate human cloning. Aeroponic culturing revolutionized cloning (vegetative propagation) from cuttings of plants. This would have been the first major breakthrough in human cloning. This changed dramatically with the advent of molecular cloning methods. There are a variety of ethical positions regarding the possibilities of cloning, especially human cloning. The various forms of cloning, particularly human cloning, are controversial. Triclosan has been employed as a selective agent in molecular cloning. These are just some of many concerns people have with animal cloning. Thus, JM109 is useful for cloning and expression systems. There is a lot of ethical debate over whether or not cloning should be used. The possibility of person cloning has raised controversies.