All splicing techniques involve installing an enclosure that protects the splice. SR proteins are important for selecting splice sites for alternative splicing. Protein coding RNAs in mitochondria are spliced and edited using organelle-specific splice and editing sites. Exon shuffling follows certain splice frame rules. An intron is separated from its exon by means of the splice site. Identified transcript variants arise from mutation at consensus splice sites. This type of sequencing is useful to capture isoforms and splice variants. Splice site mutations can be analyzed using information theory. Insertion of splice sites allows for the removal of the ITRs from the transcript. Several genetic diseases may be the result of splice site mutations. This also increases expression of the splice variant Delta FosB. This would also increase expression of the splice variant Delta FosB. However, the protein is 2818 amino acids long leading to the concept of splice variants. For applications that demand a permanent connection a fusion splice is common. Marker, an aspiring recording engineer, was learning how to splice audio tape.