Likewise the solvated protein also exists as several conformers in equilibrium. Alkane conformers are one of the subjects of alkane stereochemistry. Although conformers can be referred to as stereoisomers, they are rarely isolable in pure form. Alkane conformers arise from rotation around sp hybridised carbon carbon sigma bonds. The interconversion of chair conformers is called ring flipping or chair-flipping. Like all cyclohexanes, it can interconvert rapidly between two chair conformers. There are both steric and electronic effects that affect the relative stability of conformers. There are three possible conformers&colon; an unstacked form and two stacked forms. In methylcyclohexane the two chair conformers are not isoenergetic. When cooled to cryogenic temperatures it can have different conformers, gauche and trans. They are structurally and energetically equivalent, and are called the "staggered conformers". A limited number of (rapidly interchanging) conformers exist for this type of compound. For each diastereomer, two chair conformers are possible. Notably, all these conformers are somewhat different and present distinct features.