Cell division then occurs, and binucleate trophozoites are formed. The plasma membranes fuse to form a binucleate cell with nuclear fusion quickly following. The volume of the binucleate cell then increases to produce a giant binuclear cell. Typically "Kraken" has only one nucleus, thought binucleate organisms have been observed. This amount is similar to ciliates, but ciliates tend to be binucleate. "Sappinia" species are naked, lobose amoebe that are generally binucleate. In "Ephedra nevadensis", a single binucleate sperm cell is deposited into the egg cell. Staining with Cresyl blue shows them to be somewhat metachromatic, and binucleate. On microscopic examination, they may have one or more cytoplasmic projections and/or be binucleate. Additionally, in acetocarmine stain, they appear binucleate (having two nuclei).