Predicting and controlling the order of elution is a key aspect of column chromatographic methods. A subsequent elution with ammonium hydroxide and recrystallization from alcohol results in pure muscimol. There are distinct differences between displacement and elution chromatography. ELSDs analyze solvent after elution from HPLC. Any interaction leads to a later elution time and thus mimics a smaller analyte size. In gradient elution, the elution order may change as the dimensions or flow rate change. This type of elution is called gradient elution. First, the salt concentration in the elution buffer is gradually increased. The column is put in a centrifuge again, forcing the elution buffer through the membrane. Then, the elution of the enzyme with the appropriate substrate can be done. In elution mode, substances typically emerge from a column in narrow, Gaussian peaks. The larger the particles, the faster the elution. The mode used for elution (expanded-bed versus settled-bed) depends on the characteristics of the feed. Nonetheless, it has drawbacks such as low resolution, low capacity and low elution times. Elution curves, therefore, resemble Gaussian distributions.