Alternating between tightening and loosening can free especially stubborn lug nuts. The head's tension can be adjusted by loosening or tightening the rods. The pegs are used to tune the cello by either tightening or loosening the string. This pin prevents the nut from turning and loosening. This causes the joint to become slightly misaligned over time due to loosening bolts. Their threads are designed to induce this friction to keep the fastener from loosening. This led to the bolts loosening and failing in shear. This removes the positive charge, thus loosening the DNA from the histone. Some aluminum wheels have preventive measures against loosening with the axle. Yet newer fixtures eventually were introduced with connections designed to avoid loosening and overheating. Copper has excellent creep characteristics that minimizes loosening at connections. Gabbard introduced a measure to help food truck vendors by loosening parking restrictions. The convention ultimately compromised by loosening suffrage requirements. Tokayev supports a culture of opposition, public assembly, and loosening rules on forming political parties. He is credited as having played a crucial role in loosening regulations on campaign spending.