ORF1 encodes for nonstructural proteins and for VP1, the main capsid protein. The genome contains RNA to encode both structural and nonstructural proteins. The virus consists of four nonstructural proteins and three structural proteins. The early region encodes nonstructural proteins E1 to E7. A frame-shifting mechanism between these two nonstructural polypeptides translates RNA-dependent RNA polymerase. This group of viruses expresses structural proteins separately from the nonstructural ones. A number of the nonstructural replication proteins coalesce to form a multi-protein replicase-transcriptase complex (RTC). A number of the nonstructural proteins coalesce to form a multi-protein replicase-transcriptase complex (RTC). Ground shaking can result in both structural and nonstructural damage to buildings and other structures. The carboxyl-half of the rotavirus nonstructural protein NSP1 is not required for virus replication. The rotavirus nonstructural protein NSP4 was the first viral enterotoxin discovered. The polyprotein has its own proteases which cleave the polyprotein into multiple nonstructural proteins. The other nonstructural proteins in the complex assist in the replication and transcription process. NS codes for two nonstructural proteins (NS1 and NEP). However the pathogenicity of H5N1/97 was related to the nonstructural (NS) gene.