Another method of producing the haploids is wide crossing. After parent haploids mate, they produce diploids. Artificial production of doubled haploids is important in plant breeding. Shipra Guha-Mukherjee was an expert in plant tissue culture, haploids and plant biotechnology. Subsequently, haploids were reported in many other species. Doubled haploids can be produced "in vivo" or "in vitro". The haploid embryo is rescued, cultured, and chromosome-doubling produces doubled haploids. In its vegetative motile state, cells divide through mitosis as haploids through longitudinal fission.