Estrogen suppresses osteoclastogenesis through the upregulation of OPG expression in osteoblast lineage cells. The brand new bone created by the osteoblast is called osteoid. The active osteoblast produces substantial collagen type I. RUNX2 is a key transcription factor associated with osteoblast differentiation. These functions are critical for osteoblast proliferation and maintenance. Similar functions are seen in neuronal and osteoblast differentiation. This response might be important in maintaining osteoblast survival under some conditions. These alloys were found to cause neoplastic transformations of human osteoblast cells. Osterix (Osx) acts downstream of Runx2 and serves as a marker for normal osteoblast differentiation. First, the osteoblast puts up collagen fibers. When the osteoblast becomes trapped, it becomes known as an osteocyte. During STS-80 osteoblast cultures were grown in microgravity, using specialized hardware. The cellular dynamics of Runx2 protein are also important for proper osteoblast differentiation. OPG expression in osteoblast lineage cells is highly regulated by estrogens such as estradiol (E2). Overexpression of Notch signaling inhibits bone morphogenetic protein2-induced osteoblast differentiation.