Wnt signaling induces differentiation of pluripotent stem cells into mesoderm and endoderm progenitor cells. Gastrulation establishes the three germ layers&colon; ectoderm, mesoderm and endoderm. The germ layers are referred to as the ectoderm, mesoderm and endoderm. The endoderm and mesoderm form due to the nodal signaling. Canonical Wnt and Delta-Notch signaling progressively segregate progressive endoderm and mesoderm. An increase in expression of less than 2-fold causes differentiation into primitive endoderm and mesoderm. Each structure originates from a different germ layer&colon; the ectoderm, endoderm and mesenchyme. Unlike bilaterians, radiata only have two primordial cell layers, endoderm and ectoderm. In deuterostomes, the mesoderm forms by enterocoelic pouching, through invagination of the endoderm. It develops into the endoderm and mesoderm of an animal. The eardrum is made up of three layers (ectoderm, endoderm and connective tissue). They are diploblastic, having only two main germ layers, ectoderm and endoderm. The outer layer is known technically as the ectoderm, the inner layer as the endoderm. It can be found in endoderm or ectoderm. Parafollicular cells are derived from pharyngeal endoderm.