Methanol is used as a destaining agent in polyacrylamide gel electrophoresis. The types of gel most typically used are agarose and polyacrylamide gels. These could be fractionated by electrophoresis on a polyacrylamide gel and visualised using autoradiography. Isolated RNA is run on a denaturing polyacrylamide gel. These can be run in either agarose or polyacrylamide gels. Isoelectric focusing is also the first step in 2-D gel polyacrylamide gel electrophoresis. This method can be used with all polyacrylamide and agarose gels. Run both samples side by side on a polyacrylamide gel electrophoresis. Common synthetic polymeric nanoparticles include polyacrylamide, polyacrylate, and chitosan. It has been tested and recommended for polyacrylamide gel electrophoresis. Typical chemicals used include cationic starch and polyacrylamide (PAM) derivatives. This occurs through an insoluble matrix such as chromatographic medium like cellulose or polyacrylamide. Linear polyacrylamide is a water-soluble polymer. The more neutral pH slows the hydrolysis and thus the decomposition of the polyacrylamide. See the "Chain termination method" page for an example of a polyacrylamide DNA sequencing gel.