{"action":"created","issue":{"url":"https://api.github.com/repos/cortex-lab/Suite2P/issues/98","repository_url":"https://api.github.com/repos/cortex-lab/Suite2P","labels_url":"https://api.github.com/repos/cortex-lab/Suite2P/issues/98/labels{/name}","comments_url":"https://api.github.com/repos/cortex-lab/Suite2P/issues/98/comments","events_url":"https://api.github.com/repos/cortex-lab/Suite2P/issues/98/events","html_url":"https://github.com/cortex-lab/Suite2P/issues/98","id":264699993,"number":98,"title":"Using mRuby channel to register GCaMP channel","user":{"login":"CruzMartinLab","id":32712777,"avatar_url":"https://avatars0.githubusercontent.com/u/32712777?v=4","gravatar_id":"","url":"https://api.github.com/users/CruzMartinLab","html_url":"https://github.com/CruzMartinLab","followers_url":"https://api.github.com/users/CruzMartinLab/followers","following_url":"https://api.github.com/users/CruzMartinLab/following{/other_user}","gists_url":"https://api.github.com/users/CruzMartinLab/gists{/gist_id}","starred_url":"https://api.github.com/users/CruzMartinLab/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/CruzMartinLab/subscriptions","organizations_url":"https://api.github.com/users/CruzMartinLab/orgs","repos_url":"https://api.github.com/users/CruzMartinLab/repos","events_url":"https://api.github.com/users/CruzMartinLab/events{/privacy}","received_events_url":"https://api.github.com/users/CruzMartinLab/received_events","type":"User","site_admin":false},"labels":[],"state":"open","locked":false,"assignee":null,"assignees":[],"milestone":null,"comments":0,"created_at":"2017-10-11T19:04:37Z","updated_at":"2017-10-11T22:07:17Z","closed_at":null,"author_association":"NONE","body":"Hello,\r\n\r\nI am new to this software. We are using mRuby-P2A-GCaMP6 to visualize the activity of neurons in prefrontal cortex. In our experiments we have a really bright red channel (mRuby, cytoplasmic fill, but no activity) that we would like to use to register that same channel and a second channel that has the GCaMP signal. In the experiment the green and red channel are in the same cell. Is it possible to do this with this software?\r\n\r\nThanks in advance."},"comment":{"url":"https://api.github.com/repos/cortex-lab/Suite2P/issues/comments/335963055","html_url":"https://github.com/cortex-lab/Suite2P/issues/98#issuecomment-335963055","issue_url":"https://api.github.com/repos/cortex-lab/Suite2P/issues/98","id":335963055,"user":{"login":"hwpdalgleish","id":15194455,"avatar_url":"https://avatars2.githubusercontent.com/u/15194455?v=4","gravatar_id":"","url":"https://api.github.com/users/hwpdalgleish","html_url":"https://github.com/hwpdalgleish","followers_url":"https://api.github.com/users/hwpdalgleish/followers","following_url":"https://api.github.com/users/hwpdalgleish/following{/other_user}","gists_url":"https://api.github.com/users/hwpdalgleish/gists{/gist_id}","starred_url":"https://api.github.com/users/hwpdalgleish/starred{/owner}{/repo}","subscriptions_url":"https://api.github.com/users/hwpdalgleish/subscriptions","organizations_url":"https://api.github.com/users/hwpdalgleish/orgs","repos_url":"https://api.github.com/users/hwpdalgleish/repos","events_url":"https://api.github.com/users/hwpdalgleish/events{/privacy}","received_events_url":"https://api.github.com/users/hwpdalgleish/received_events","type":"User","site_admin":false},"created_at":"2017-10-11T22:07:17Z","updated_at":"2017-10-11T22:07:17Z","author_association":"CONTRIBUTOR","body":"Hey,\r\n\r\nBy register, do you mean on a given day register all frames for the dataset for motion correction? Or are you talking about registering entire movies across multiple days to track cells over time?\r\n\r\nHenry\r\n"}}
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