<P> The formation of ATP from ADP and P is energetically unfavorable and would normally proceed in the reverse direction . In order to drive this reaction forward , ATPase couples ATP synthesis during cellular respiration to an electrochemical gradient created by the difference in proton ( H ) concentration across the mitochondrial membrane in eukaryotes or the plasma membrane in bacteria . During photosynthesis in plants , ATP is synthesized by ATPase using a proton gradient created in the thylakoid lumen through the thylakoid membrane and into the chloroplast stroma . </P>
where does the formation of atp take place
mitochondrial membrane in eukaryotes plasma membrane in bacteria