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DNA isolation
All solutions used for DNA isolation are purged with nitrogen (immediately prior to use) and supplemented with 50 muM phenyl-tert-butyl nitrone to minimise oxidative damage to DNA[31].
Qiagen DNAeasy Kit (#69506)
includes: Proteinase K, AW1, AW2, AE and Spin-columns
Dithiothreitol (DTT; Sigma)
Ethanol (Ajax Finechem #214-2.5L)
Nitrogen (for purging of buffers)
