successful techniques synthetically producing proteins contain phosphomimetic molecules either terminus ref name pmid addition researchers used unnatural amino acid mutagenesis targeted sites within peptide sequence ref name pmid ref advances chemical biology also improved upon classical techniques imaging kinase action example development peptide biosensors peptides containing incorporated fluorophores improved temporal resolution vitro binding assays ref name pmid ref one useful techniques study kinase action fluorescence resonance energy transfer fret utilize fret phosphorylation studies fluorescent proteins coupled phosphoamino acid binding domain
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