require blocking prevent undesired side reactions blocking functional groups well subsequent de blocking groups coupling subsequent nucleotides eventual cleaving solid support ref name ocorr methods manipulation biomolecules attributed biomolecular engineering increase interleukin levels directly proportional increased death rate breast cancer patients pcr paired western blotting elisa help define relationship cancer cells il enzyme linked immunosorbent assay elisa enzyme linked immunosorbent assay assay utilizes principle antibody antigen recognition
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