n terminal cysteine residue ultimately resulting formation native amide bond strategies used ligation peptide fragments using acyl transfer chemistry first introduced native chemical ligation include expressed protein ligation ref name pmid ref sulfurization desulfurization techniques ref name pmid ref use removable thiol auxiliaries ref name pmid ref expressed protein ligation allows biotechnological installation c terminal thioester using inteins thereby allowing appendage synthetic n terminal peptide recombinantly produced c terminal portion sulfurization desulfurization techniques use removable thiol auxiliaries involve installation synthetic thiol moiety carry
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