Using post-embedding immunogold electron microscopy, TAR DNA-binding protein of 43 kDa (TDP-43) was localized to neuronal cytoplasmic (NCI) and intranuclear (NII) inclusions, as well as unmyelinated neurites, in frontotemporal lobar degeneration with ubiquitinated inclusions (FTLD-U), amyotrophic lateral sclerosis (ALS), Alzheimer's (AD), Pick's disease (PiD) and Lewy body disease (LBD). The TDP-43 immunoreactive structures were morphologically heterogeneous. The most common was characterized by bundles of 10-20 nm diameter straight filaments with electron dense granular material within NCI, NII and neurites. This type of pathology was found in FTLD-U, ALS and some cases of AD. Less often, inclusions in neuritic processes of FTLD-U and some cases of AD contained 10-17 nm diameter straight filaments without granular material. A final type of TDP-43 immunoreactivity was labeling of filaments and granular material associated with tau filaments in neurofibrillary tangles of AD and Pick bodies of PiD or alpha-synuclein filaments in Lewy bodies of LBD. The results suggest that TDP-43 is the primary component of the granulofilamentous inclusions in FTLD-U and ALS. Similar inclusions sometimes accompany filamentous aggregates composed of other abnormal proteins in AD, PiD and LBD.
Using post - embedding immunogold electron microscopy , TAR DNA - binding protein of 43 kDa ( TDP-43 ) was localized to neuronal cytoplasmic ( NCI ) and intranuclear ( NII ) inclusions , as well as unmyelinated neurites , in frontotemporal lobar degeneration with ubiquitinated inclusions ( FTLD - U ) , amyotrophic lateral sclerosis ( ALS ) , Alzheimer 's ( AD ) , Pick 's disease ( PiD ) and Lewy body disease ( LBD ) . The TDP-43 immunoreactive structures were morphologically heterogeneous . The most common was characterized by bundles of 10 - 20 nm diameter straight filaments with electron dense granular material within NCI , NII and neurites . This type of pathology was found in FTLD - U , ALS and some cases of AD . Less often , inclusions in neuritic processes of FTLD - U and some cases of AD contained 10 - 17 nm diameter straight filaments without granular material . A final type of TDP-43 immunoreactivity was labeling of filaments and granular material associated with tau filaments in neurofibrillary tangles of AD and Pick bodies of PiD or alpha - synuclein filaments in Lewy bodies of LBD . The results suggest that TDP-43 is the primary component of the granulofilamentous inclusions in FTLD - U and ALS . Similar inclusions sometimes accompany filamentous aggregates composed of other abnormal proteins in AD , PiD and LBD .
Which is the primary protein component of Lewy bodies?
Which is the primary protein component of Lewy bodies ?
alpha-synuclein
alpha-synuclein α-synuclein αSyn