alpha-Synuclein is a presynaptic protein recently identified as a specific component of Lewy bodies (LB) and Lewy neurites. The aim of this study was to assess the morphology and distribution of alpha-synuclein immunoreactivity in cases of dementia with LB (DLB), and to compare alpha-synuclein with ubiquitin immunostaining. We examined substantia nigra, paralimbic regions (entorhinal cortex, cingulate gyrus, insula and hippocampus), and neocortex (frontal and occipital association cortices) with double alpha-synuclein and ubiquitin immunostaining in 25 cases meeting neuropathological criteria for DLB. alpha-Synuclein immunostaining was more specific than ubiquitin immunostaining in that it differentiated LB from globose tangles. It was also slightly more sensitive, staining 4-5% more intracytoplasmic structures, especially diffuse alpha-synuclein deposits that were ubiquitin negative. In addition to LB, alpha-synuclein staining showed filiform and globose neurites in the substantia nigra, CA2-3 regions of the hippocampus, and entorhinal cortex. A spectrum of alpha-synuclein staining was seen in substantia nigra: from diffuse "cloud-like" inclusions to aggregated intracytoplasmic inclusions with variable ubiquitin staining to classic LB. We hypothesize that these represent different stages in LB formation.
alpha - Synuclein is a presynaptic protein recently identified as a specific component of Lewy bodies ( LB ) and Lewy neurites . The aim of this study was to assess the morphology and distribution of alpha - synuclein immunoreactivity in cases of dementia with LB ( DLB ) , and to compare alpha - synuclein with ubiquitin immunostaining . We examined substantia nigra , paralimbic regions ( entorhinal cortex , cingulate gyrus , insula and hippocampus ) , and neocortex ( frontal and occipital association cortices ) with double alpha - synuclein and ubiquitin immunostaining in 25 cases meeting neuropathological criteria for DLB . alpha - Synuclein immunostaining was more specific than ubiquitin immunostaining in that it differentiated LB from globose tangles . It was also slightly more sensitive , staining 4 - 5 % more intracytoplasmic structures , especially diffuse alpha - synuclein deposits that were ubiquitin negative . In addition to LB , alpha - synuclein staining showed filiform and globose neurites in the substantia nigra , CA2 - 3 regions of the hippocampus , and entorhinal cortex . A spectrum of alpha - synuclein staining was seen in substantia nigra : from diffuse " cloud - like " inclusions to aggregated intracytoplasmic inclusions with variable ubiquitin staining to classic LB . We hypothesize that these represent different stages in LB formation .
Against which protein is the antibody used for immonostaining of Lewy bodies raised?
Against which protein is the antibody used for immonostaining of Lewy bodies raised ?
alpha-Synuclein
alpha-Synuclein