Many studies have reported that the generation of reactive oxygen species (ROS) increases during the differentiation of muscle-derived C2C12 cells. Peroxiredoxin-2 (Prx-2) is an abundant mammalian enzyme that protects against oxidative stress. However, the role of Prx-2 in muscle differentiation has not been investigated. In this study, we demonstrated that Prx-2 expression increases during muscle differentiation and regeneration in response to exogenous H(2)O(2). This increase occurs only in myoblast cell lines because no increase in Prx-2 expression was observed in the NIH3T3, MEF, Chang, or HEK293 cell lines. The antioxidants, N-acetyl L-cysteine (NAC) and 4,5-dihydroxy-1,3-benzenedisulfonic acid (Tiron), both suppressed myogenesis and Prx-2 expression. Moreover, Prx-2 was upregulated at the transcriptional level by NF-κB during the differentiation of muscle-derived C2C12 cells. We also found that inhibition of phosphatidylinositol 3-kinase (PI3K) blocks NF-κB activation and suppresses Prx-2 expression. Interestingly, Prx-2 knockdown increased the expression levels of other antioxidant enzymes, including all of the other Prx family member, thioredoxin-1 (Trx-1) and catalase, but also enhanced the accumulation of endogenous ROS during muscle differentiation. In this study, we demonstrated for the first time that Prx-2 is unregulated during the muscle differentiation and regeneration. Prx-2 is upregulated via the PI3K/NF-κB pathway and attenuates oxidative stress during muscle differentiation and regeneration.
Many studies have reported that the generation of reactive oxygen species ( ROS ) increases during the differentiation of muscle - derived C2C12 cells . Peroxiredoxin-2 ( Prx-2 ) is an abundant mammalian enzyme that protects against oxidative stress . However , the role of Prx-2 in muscle differentiation has not been investigated . In this study , we demonstrated that Prx-2 expression increases during muscle differentiation and regeneration in response to exogenous H(2)O(2 ) . This increase occurs only in myoblast cell lines because no increase in Prx-2 expression was observed in the NIH3T3 , MEF , Chang , or HEK293 cell lines . The antioxidants , N - acetyl L - cysteine ( NAC ) and 4,5-dihydroxy-1,3-benzenedisulfonic acid ( Tiron ) , both suppressed myogenesis and Prx-2 expression . Moreover , Prx-2 was upregulated at the transcriptional level by NF - κB during the differentiation of muscle - derived C2C12 cells . We also found that inhibition of phosphatidylinositol 3-kinase ( PI3 K ) blocks NF - κB activation and suppresses Prx-2 expression . Interestingly , Prx-2 knockdown increased the expression levels of other antioxidant enzymes , including all of the other Prx family member , thioredoxin-1 ( Trx-1 ) and catalase , but also enhanced the accumulation of endogenous ROS during muscle differentiation . In this study , we demonstrated for the first time that Prx-2 is unregulated during the muscle differentiation and regeneration . Prx-2 is upregulated via the PI3K / NF - κB pathway and attenuates oxidative stress during muscle differentiation and regeneration .
What type of enzyme is peroxiredoxin 2 (PRDX2)?
What type of enzyme is peroxiredoxin 2 ( PRDX2 ) ?
antioxidant
antioxidant