The t(9;22)(q34;q11) translocation leading to the Philadelphia (Ph) chromosome resulting in BCR-ABL gene fusion is associated with a poor prognosis in acute lymphoblastic leukemia (ALL). We studied the relation between t(9;22), determined by karyotype, fluorescence in situ hybridization (FISH) or polymerase chain reaction (PCR), and in vitro drug resistance, measured by the MTT assay, in precursor B-cell ALL at diagnosis. The findings in twenty-one Ph-positive (Ph+) childhood common/precursorB (c/preB) cases were compared with 254 Ph-negative (Ph-) ALL cases. A large range of LC(50) values was found within the Ph+ patients. Moreover, LC(50) values did not differ significantly between Ph+ and Ph- samples for prednisolone, dexamethasone, L-asparaginase, vincristine, anthracyclines, thiopurines, epipodophyllotoxins, and 4H00-ifosfamide, even after matching for important prognostic features (age, white blood cell count (WBC), and immunophenotype). Adult Ph+ (n = 12) ALL was more resistant to prednisolone (> 270-fold, P = 0.030), and displayed an overall tendency to resistance when compared to matched cases of Ph- (n = 15) adult precursor B-cell ALL. Within Ph+ ALL, in vitro prednisolone resistance increased significantly with age (P = 0.006). The expression of lung resistance protein (LRP), but not P-glycoprotein (P-gp) or multidrug resistance protein (MRP), was significantly higher in all Ph+ patients. Both childhood and adult Ph+ precursor B-cell ALL samples display a heterogeneous in vitro resistance profile, with relatively sensitive and resistant cases. The adult Ph+ samples, however, are generally more resistant compared to matched Ph- controls, reaching significance for prednisolone. The correlation of prednisolone resistance with age within the Ph+ cases might help explain the poorer prognosis of adult Ph+ ALL.
The t(9;22)(q34;q11 ) translocation leading to the Philadelphia ( Ph ) chromosome resulting in BCR - ABL gene fusion is associated with a poor prognosis in acute lymphoblastic leukemia ( ALL ) . We studied the relation between t(9;22 ) , determined by karyotype , fluorescence in situ hybridization ( FISH ) or polymerase chain reaction ( PCR ) , and in vitro drug resistance , measured by the MTT assay , in precursor B - cell ALL at diagnosis . The findings in twenty - one Ph - positive ( Ph+ ) childhood common / precursorB ( c / preB ) cases were compared with 254 Ph - negative ( Ph- ) ALL cases . A large range of LC(50 ) values was found within the Ph+ patients . Moreover , LC(50 ) values did not differ significantly between Ph+ and Ph- samples for prednisolone , dexamethasone , L - asparaginase , vincristine , anthracyclines , thiopurines , epipodophyllotoxins , and 4H00-ifosfamide , even after matching for important prognostic features ( age , white blood cell count ( WBC ) , and immunophenotype ) . Adult Ph+ ( n = 12 ) ALL was more resistant to prednisolone ( > 270-fold , P = 0.030 ) , and displayed an overall tendency to resistance when compared to matched cases of Ph- ( n = 15 ) adult precursor B - cell ALL . Within Ph+ ALL , in vitro prednisolone resistance increased significantly with age ( P = 0.006 ) . The expression of lung resistance protein ( LRP ) , but not P - glycoprotein ( P - gp ) or multidrug resistance protein ( MRP ) , was significantly higher in all Ph+ patients . Both childhood and adult Ph+ precursor B - cell ALL samples display a heterogeneous in vitro resistance profile , with relatively sensitive and resistant cases . The adult Ph+ samples , however , are generally more resistant compared to matched Ph- controls , reaching significance for prednisolone . The correlation of prednisolone resistance with age within the Ph+ cases might help explain the poorer prognosis of adult Ph+ ALL .
Which gene fusion is the result of the "philadelphia translocation" or the "philadelphia chromosome" mutation?
Which gene fusion is the result of the " philadelphia translocation " or the " philadelphia chromosome " mutation ?
The BCR/ABL gene fusion
The BCR/ABL gene fusion ABL/BCR fusion