It is well established that growth-factor-induced reactive oxygen species (ROS) act as second messengers in cell signaling. We have previously reported that betaPix, a guanine nucleotide exchange factor for Rac, interacts with NADPH oxidase 1 (Nox1) leading to EGF-induced ROS generation. Here, we report the identification of the domains of Nox1 and betaPix responsible for the interaction between the two proteins. GST pull-down assays show that the PH domain of betaPix binds to the FAD-binding region of Nox1. We also show that overexpression of the PH domain of betaPix results in inhibition of superoxide anion generation in response to EGF. Additionally, NADPH oxidase Organizer 1 (NoxO1) is shown to interact with the NADPH-binding region of Nox1. These results suggest that the formation of the complex consisting of Nox1, betaPix, and NoxO1 is likely to be a critical step in EGF-induced ROS generation.
It is well established that growth - factor - induced reactive oxygen species ( ROS ) act as second messengers in cell signaling . We have previously reported that betaPix , a guanine nucleotide exchange factor for Rac , interacts with NADPH oxidase 1 ( Nox1 ) leading to EGF - induced ROS generation . Here , we report the identification of the domains of Nox1 and betaPix responsible for the interaction between the two proteins . GST pull - down assays show that the PH domain of betaPix binds to the FAD - binding region of Nox1 . We also show that overexpression of the PH domain of betaPix results in inhibition of superoxide anion generation in response to EGF . Additionally , NADPH oxidase Organizer 1 ( NoxO1 ) is shown to interact with the NADPH - binding region of Nox1 . These results suggest that the formation of the complex consisting of Nox1 , betaPix , and NoxO1 is likely to be a critical step in EGF - induced ROS generation .
Which NADPH oxidase family member requires interaction with NOXO1 for function?
Which NADPH oxidase family member requires interaction with NOXO1 for function ?
NADPH oxidase 1 NOX1
NADPH oxidase 1 NOX1 nicotinamide adenine dinucleotide phosphate-oxidase 1