The precise regulation of microRNA (miRNA) transcription and processing is important for eukaryotic development. Plant miRNAs are first transcribed as stem-loop primary miRNAs (pri-miRNAs) by RNA polymerase II,then cleaved in the nucleus into mature miRNAs by Dicer-like 1 (DCL1). We identified a cycling DOF transcription factor, CDF2, which interacts with DCL1 and regulates the accumulation of a population of miRNAs. CDF2 binds directly to the promoters of some miRNAs and works as a transcription activator or repressor for these miRNA genes. CDF2 binds preferentially to the pri-miRNAs regulated by itself and affects DCL1-mediated processing of these pri-miRNAs. Genetically, CDF2 works in the same pathway as miR156 or miR172 to control flowering. We conclude that CDF2 regulates a group of pri-miRNAs at both the transcriptional and posttranscriptional levels to maintain proper levels of their mature miRNAs to control plant development.
The precise regulation of microRNA ( miRNA ) transcription and processing is important for eukaryotic development . Plant miRNAs are first transcribed as stem - loop primary miRNAs ( pri - miRNAs ) by RNA polymerase II , then cleaved in the nucleus into mature miRNAs by Dicer - like 1 ( DCL1 ) . We identified a cycling DOF transcription factor , CDF2 , which interacts with DCL1 and regulates the accumulation of a population of miRNAs . CDF2 binds directly to the promoters of some miRNAs and works as a transcription activator or repressor for these miRNA genes . CDF2 binds preferentially to the pri - miRNAs regulated by itself and affects DCL1-mediated processing of these pri - miRNAs . Genetically , CDF2 works in the same pathway as miR156 or miR172 to control flowering . We conclude that CDF2 regulates a group of pri - miRNAs at both the transcriptional and posttranscriptional levels to maintain proper levels of their mature miRNAs to control plant development .
Which polymerase transcribes pri-miRNAs?
Which polymerase transcribes pri - miRNAs ?
RNA polymerase II
RNA polymerase II RNAPII