All archaeal and many bacterial genomes contain Clustered Regularly Interspaced Short Palindrome Repeats (CRISPR) and variable arrays of the CRISPR-associated (cas) genes that have been previously implicated in a novel form of DNA repair on the basis of comparative analysis of their protein product sequences. However, the proximity of CRISPR and cas genes strongly suggests that they have related functions which is hard to reconcile with the repair hypothesis. The protein sequences of the numerous cas gene products were classified into approximately 25 distinct protein families; several new functional and structural predictions are described. Comparative-genomic analysis of CRISPR and cas genes leads to the hypothesis that the CRISPR-Cas system (CASS) is a mechanism of defense against invading phages and plasmids that functions analogously to the eukaryotic RNA interference (RNAi) systems. Specific functional analogies are drawn between several components of CASS and proteins involved in eukaryotic RNAi, including the double-stranded RNA-specific helicase-nuclease (dicer), the endonuclease cleaving target mRNAs (slicer), and the RNA-dependent RNA polymerase. However, none of the CASS components is orthologous to its apparent eukaryotic functional counterpart. It is proposed that unique inserts of CRISPR, some of which are homologous to fragments of bacteriophage and plasmid genes, function as prokaryotic siRNAs (psiRNA), by base-pairing with the target mRNAs and promoting their degradation or translation shutdown. Specific hypothetical schemes are developed for the functioning of the predicted prokaryotic siRNA system and for the formation of new CRISPR units with unique inserts encoding psiRNA conferring immunity to the respective newly encountered phages or plasmids. The unique inserts in CRISPR show virtually no similarity even between closely related bacterial strains which suggests their rapid turnover, on evolutionary scale. Corollaries of this finding are that, even among closely related prokaryotes, the most commonly encountered phages and plasmids are different and/or that the dominant phages and plasmids turn over rapidly. We proposed previously that Cas proteins comprise a novel DNA repair system. The association of the cas genes with CRISPR and, especially, the presence, in CRISPR units, of unique inserts homologous to phage and plasmid genes make us abandon this hypothesis. It appears most likely that CASS is a prokaryotic system of defense against phages and plasmids that functions via the RNAi mechanism. The functioning of this system seems to involve integration of fragments of foreign genes into archaeal and bacterial chromosomes yielding heritable immunity to the respective agents. However, it appears that this inheritance is extremely unstable on the evolutionary scale such that the repertoires of unique psiRNAs are completely replaced even in closely related prokaryotes, presumably, in response to rapidly changing repertoires of dominant phages and plasmids.
All archaeal and many bacterial genomes contain Clustered Regularly Interspaced Short Palindrome Repeats ( CRISPR ) and variable arrays of the CRISPR - associated ( cas ) genes that have been previously implicated in a novel form of DNA repair on the basis of comparative analysis of their protein product sequences . However , the proximity of CRISPR and cas genes strongly suggests that they have related functions which is hard to reconcile with the repair hypothesis . The protein sequences of the numerous cas gene products were classified into approximately 25 distinct protein families ; several new functional and structural predictions are described . Comparative - genomic analysis of CRISPR and cas genes leads to the hypothesis that the CRISPR - Cas system ( CASS ) is a mechanism of defense against invading phages and plasmids that functions analogously to the eukaryotic RNA interference ( RNAi ) systems . Specific functional analogies are drawn between several components of CASS and proteins involved in eukaryotic RNAi , including the double - stranded RNA - specific helicase - nuclease ( dicer ) , the endonuclease cleaving target mRNAs ( slicer ) , and the RNA - dependent RNA polymerase . However , none of the CASS components is orthologous to its apparent eukaryotic functional counterpart . It is proposed that unique inserts of CRISPR , some of which are homologous to fragments of bacteriophage and plasmid genes , function as prokaryotic siRNAs ( psiRNA ) , by base - pairing with the target mRNAs and promoting their degradation or translation shutdown . Specific hypothetical schemes are developed for the functioning of the predicted prokaryotic siRNA system and for the formation of new CRISPR units with unique inserts encoding psiRNA conferring immunity to the respective newly encountered phages or plasmids . The unique inserts in CRISPR show virtually no similarity even between closely related bacterial strains which suggests their rapid turnover , on evolutionary scale . Corollaries of this finding are that , even among closely related prokaryotes , the most commonly encountered phages and plasmids are different and/or that the dominant phages and plasmids turn over rapidly . We proposed previously that Cas proteins comprise a novel DNA repair system . The association of the cas genes with CRISPR and , especially , the presence , in CRISPR units , of unique inserts homologous to phage and plasmid genes make us abandon this hypothesis . It appears most likely that CASS is a prokaryotic system of defense against phages and plasmids that functions via the RNAi mechanism . The functioning of this system seems to involve integration of fragments of foreign genes into archaeal and bacterial chromosomes yielding heritable immunity to the respective agents . However , it appears that this inheritance is extremely unstable on the evolutionary scale such that the repertoires of unique psiRNAs are completely replaced even in closely related prokaryotes , presumably , in response to rapidly changing repertoires of dominant phages and plasmids .
Gene silencing can be achieved by RNA interference (RNAi) in eukaryotic organisms. What is the name of the analogous process in prokaryotic organisms?
Gene silencing can be achieved by RNA interference ( RNAi ) in eukaryotic organisms . What is the name of the analogous process in prokaryotic organisms ?
CRISPR-Cas
CRISPR-Cas