The antennal lobe (primary olfactory center of insects) is completely reorganized during metamorphosis. This reorganization is accompanied by changing patterns of calcium signaling in neurons and glial cells. In the present study, we investigated the developmental distribution of a major calcium-dependent protein, viz., calcium/calmodulin-dependent protein kinase II (CaM kinase II), in the antennal lobe of the sphinx moth Manduca sexta by using a monoclonal antibody. During synaptogenesis (developmental stages 6-10), we found a redistribution of CaM kinase II immunoreactivity, from a homogeneous distribution in the immature neuropil to an accumulation in the neuropil of the glomeruli. CaM kinase II immunoreactivity was less intense in olfactory receptor axons of the antennal nerve and antennal lobe glial cells. Western blot analysis revealed a growing content of CaM kinase II in antennal lobe tissue throughout metamorphosis. Injection of the CaM kinase inhibitor KN-93 into pupae resulted in a reduced number of antennal lobe glial cells migrating into the neuropil to form borders around glomeruli. The results suggest that CaM kinase II is involved in glial cell migration.
The antennal lobe ( primary olfactory center of insects ) is completely reorganized during metamorphosis . This reorganization is accompanied by changing patterns of calcium signaling in neurons and glial cells . In the present study , we investigated the developmental distribution of a major calcium - dependent protein , viz . , calcium / calmodulin - dependent protein kinase II ( CaM kinase II ) , in the antennal lobe of the sphinx moth Manduca sexta by using a monoclonal antibody . During synaptogenesis ( developmental stages 6 - 10 ) , we found a redistribution of CaM kinase II immunoreactivity , from a homogeneous distribution in the immature neuropil to an accumulation in the neuropil of the glomeruli . CaM kinase II immunoreactivity was less intense in olfactory receptor axons of the antennal nerve and antennal lobe glial cells . Western blot analysis revealed a growing content of CaM kinase II in antennal lobe tissue throughout metamorphosis . Injection of the CaM kinase inhibitor KN-93 into pupae resulted in a reduced number of antennal lobe glial cells migrating into the neuropil to form borders around glomeruli . The results suggest that CaM kinase II is involved in glial cell migration .
Which kinase is inhibited by the small molecule KN-93?
Which kinase is inhibited by the small molecule KN-93 ?
The calcium/calmodulin-dependent protein kinase-II CaM kinase II
CAMK2 calcium/calmodulin-dependent protein kinase II The calcium/calmodulin-dependent protein kinase-II CaM kinase II