The histone variant H2A.Z is a universal mark of gene promoters, enhancers, and regulatory elements in eukaryotic chromatin. The chromatin remodeler SWR1 mediates site-specific incorporation of H2A.Z by a multi-step histone replacement reaction, evicting histone H2A-H2B from the canonical nucleosome and depositing the H2A.Z-H2B dimer. Binding of both substrates, the canonical nucleosome and the H2A.Z-H2B dimer, is essential for activation of SWR1. We found that SWR1 primarily recognizes key residues within the α2 helix in the histone-fold of nucleosomal histone H2A, a region not previously known to influence remodeler activity. Moreover, SWR1 interacts preferentially with nucleosomal DNA at superhelix location 2 on the nucleosome face distal to its linker-binding site. Our findings provide new molecular insights on recognition of the canonical nucleosome by a chromatin remodeler and have implications for ATP-driven mechanisms of histone eviction and deposition.
The histone variant H2A.Z is a universal mark of gene promoters , enhancers , and regulatory elements in eukaryotic chromatin . The chromatin remodeler SWR1 mediates site - specific incorporation of H2A.Z by a multi - step histone replacement reaction , evicting histone H2A - H2B from the canonical nucleosome and depositing the H2A.Z - H2B dimer . Binding of both substrates , the canonical nucleosome and the H2A.Z - H2B dimer , is essential for activation of SWR1 . We found that SWR1 primarily recognizes key residues within the α2 helix in the histone - fold of nucleosomal histone H2A , a region not previously known to influence remodeler activity . Moreover , SWR1 interacts preferentially with nucleosomal DNA at superhelix location 2 on the nucleosome face distal to its linker - binding site . Our findings provide new molecular insights on recognition of the canonical nucleosome by a chromatin remodeler and have implications for ATP - driven mechanisms of histone eviction and deposition .
Which protein mediates the replacement of H2A by H2A.Z in the yeast Saccharomyces cerevisiae?
Which protein mediates the replacement of H2A by H2A.Z in the yeast Saccharomyces cerevisiae ?
SWR1
SWR1