We transplanted adult whole bone marrow prelabeled with bromodeoxyuridine (BrdU) into the ischemic boundary zone of the adult rat brain at 1 day after 2 h of middle cerebral artery occlusion (MCAo). Approximately 3.3% of 10(6) transplanted bone marrow cells were BrdU reactive at 14 days after MCAo. BrdU-reactive cells expressed neuronal and astrocytic proteins, neuronal nuclei protein (NeuN, 1%), and glial fibrillary acidic protein (GFAP, 5%) immunoreactivities, respectively. In addition, bone marrow transplantation promoted proliferation of ependymal and subependymal cells, identified by nestin (a neuroepithelial stem cell marker), within the ventricular zone and subventricular zone (VZ/SVZ). These data suggest that intracerebral transplantation of bone marrow could potentially be used to induce plasticity in ischemic brain.
We transplanted adult whole bone marrow prelabeled with bromodeoxyuridine ( BrdU ) into the ischemic boundary zone of the adult rat brain at 1 day after 2 h of middle cerebral artery occlusion ( MCAo ) . Approximately 3.3 % of 10(6 ) transplanted bone marrow cells were BrdU reactive at 14 days after MCAo . BrdU - reactive cells expressed neuronal and astrocytic proteins , neuronal nuclei protein ( NeuN , 1 % ) , and glial fibrillary acidic protein ( GFAP , 5 % ) immunoreactivities , respectively . In addition , bone marrow transplantation promoted proliferation of ependymal and subependymal cells , identified by nestin ( a neuroepithelial stem cell marker ) , within the ventricular zone and subventricular zone ( VZ / SVZ ) . These data suggest that intracerebral transplantation of bone marrow could potentially be used to induce plasticity in ischemic brain .
Which intermediate filament (IF) protein can be used as a non-specific marker of the neuronal precursor cells of the subventricular zone?
Which intermediate filament ( IF ) protein can be used as a non - specific marker of the neuronal precursor cells of the subventricular zone ?
Nestin
Nestin