Peroxiredoxin-2 (PRDX-2) is an antioxidant and chaperone-like protein critical for cell function. This study examined whether the levels of lymphocyte PRDX-2 are altered over 1 month following ultra-endurance exercise. Nine middle-aged men undertook a single-stage, multi-day 233 km (145 mile) ultra-endurance running race. Blood was collected immediately before (Pre), upon completion/retirement (Post), and following the race at Day 1, Day 7 and Day 28. Lymphocyte lysates were examined for PRDX-2 by reducing and non-reducing SDS-PAGE with western blotting. In a sub-group of men who completed the race (n = 4), PRDX-2 oligomeric state (indicative of redox status) was investigated. Ultra-endurance exercise caused significant changes in lymphocyte PRDX-2 (F(4,32) 3.409, p = 0.020, η(2) = 0.299): 7 days after the race, PRDX-2 levels in lymphocytes had fallen to 30% of pre-race values (p = 0.013) and returned to near-normal levels at Day 28. Non-reducing gels demonstrated that dimeric PRDX-2 (intracellular reduced PRDX-2 monomers) was increased in three of four race completers immediately post-race, indicative of an 'antioxidant response'. Moreover, monomeric PRDX-2 was also increased immediately post-race in two of four race-completing subjects, indicative of oxidative damage, which was not detectable by Day 7. Lymphocyte PRDX-2 was decreased below normal levels 7 days after ultra-endurance exercise. Excessive accumulation of reactive oxygen species induced by ultra-endurance exercise may underlie depletion of lymphocyte PRDX-2 by triggering its turnover after oxidation. Low levels of lymphocyte PRDX-2 could influence cell function and might, in part, explain reports of dysregulated immunity following ultra-endurance exercise.
Peroxiredoxin-2 ( PRDX-2 ) is an antioxidant and chaperone - like protein critical for cell function . This study examined whether the levels of lymphocyte PRDX-2 are altered over 1 month following ultra - endurance exercise . Nine middle - aged men undertook a single - stage , multi - day 233 km ( 145 mile ) ultra - endurance running race . Blood was collected immediately before ( Pre ) , upon completion / retirement ( Post ) , and following the race at Day 1 , Day 7 and Day 28 . Lymphocyte lysates were examined for PRDX-2 by reducing and non - reducing SDS - PAGE with western blotting . In a sub - group of men who completed the race ( n = 4 ) , PRDX-2 oligomeric state ( indicative of redox status ) was investigated . Ultra - endurance exercise caused significant changes in lymphocyte PRDX-2 ( F(4,32 ) 3.409 , p = 0.020 , η(2 ) = 0.299 ) : 7 days after the race , PRDX-2 levels in lymphocytes had fallen to 30 % of pre - race values ( p = 0.013 ) and returned to near - normal levels at Day 28 . Non - reducing gels demonstrated that dimeric PRDX-2 ( intracellular reduced PRDX-2 monomers ) was increased in three of four race completers immediately post - race , indicative of an ' antioxidant response ' . Moreover , monomeric PRDX-2 was also increased immediately post - race in two of four race - completing subjects , indicative of oxidative damage , which was not detectable by Day 7 . Lymphocyte PRDX-2 was decreased below normal levels 7 days after ultra - endurance exercise . Excessive accumulation of reactive oxygen species induced by ultra - endurance exercise may underlie depletion of lymphocyte PRDX-2 by triggering its turnover after oxidation . Low levels of lymphocyte PRDX-2 could influence cell function and might , in part , explain reports of dysregulated immunity following ultra - endurance exercise .
What type of enzyme is peroxiredoxin 2 (PRDX2)?
What type of enzyme is peroxiredoxin 2 ( PRDX2 ) ?
antioxidant
antioxidant