We examined the involvement of the Na(+)/Ca(2+) exchanger in the automaticity of the pulmonary vein myocardium with a specific inhibitor, SEA0400. Action potentials were recorded from the myocardial layer of isolated guinea-pig pulmonary vein preparations, and Ca(2+) transients were recorded from the cardiomyocytes. Spontaneous electrical activity was observed in 17.7% of the preparations, which was inhibited by either SEA0400 or ryanodine. In quiescent preparations, ouabain induced electrical activity and spontaneous Ca(2+) transients, which were inhibited by SEA0400, as well as ryanodine. These results provide pharmacological evidence that the Na(+)/Ca(2+) exchanger underlies the automaticity of the pulmonary vein myocardium.
We examined the involvement of the Na(+)/Ca(2 + ) exchanger in the automaticity of the pulmonary vein myocardium with a specific inhibitor , SEA0400 . Action potentials were recorded from the myocardial layer of isolated guinea - pig pulmonary vein preparations , and Ca(2 + ) transients were recorded from the cardiomyocytes . Spontaneous electrical activity was observed in 17.7 % of the preparations , which was inhibited by either SEA0400 or ryanodine . In quiescent preparations , ouabain induced electrical activity and spontaneous Ca(2 + ) transients , which were inhibited by SEA0400 , as well as ryanodine . These results provide pharmacological evidence that the Na(+)/Ca(2 + ) exchanger underlies the automaticity of the pulmonary vein myocardium .
The small molecule SEA0400 is an inhibitor of which ion antiporter/exchanger?
The small molecule SEA0400 is an inhibitor of which ion antiporter / exchanger ?
Na(+)/Ca(2+) exchanger
Na(+)/Ca(2+) exchanger NCX