hematology and oncology
Aplastic Anemia
Diagnosis of Aplastic Anemia
Complete blood count (CBC) and reticulocyte count Bone marrow examination with cytogenetic and molecular testing Flow cytometry for paroxysmal nocturnal hemoglobinuria (PNH) clone Consideration of genetic testing especially in young patients or those with suggestive family history or characteristic dysmorphia Detection of a PNH clone can exclude an inherited syndrome ( Diagnosis reference ). Aplastic anemia is suspected in patients, particularly young patients, with pancytopenia. Severe aplastic anemia is defined by a bone marrow with < 25% cellularity (hypocellularity) and the presence of ≥ 2 of the following: Absolute neutrophil count < 500/microL (< 0.5 × 109/L) Absolute reticulocyte count < 60,000/microL (< 60 × 109/L) Platelet count < 20,000/microL (< 20 × 109/L) Very severe aplastic anemia is defined as absolute neutrophil count < 200/microL (< 0.2 x 109/L). 1.DeZern AE, Symons HJ, Resar LS, Borowitz MJ, Armanios MY, Brodsky RA. Detection of paroxysmal nocturnal hemoglobinuria clones to exclude inherited bone marrow failure syndromes.Eur J Haematol2014;92(6):467-470. doi:10.1111/ejh.12299 