title: load data error (Read10X, Seurat - Guided Clustering Tutorial)
body: <p>I am trying to follow the tutorial from Seurat website.
  <a href="https://satijalab.org/seurat/v3.1/pbmc3k_tutorial.html" rel="nofollow noreferrer">https://satijalab.org/seurat/v3.1/pbmc3k_tutorial.html</a></p>
  <p>I got an error when loading the data.</p>
  <blockquote>
  <p>Load the PBMC dataset</p>
  <p>pbmc.data &lt;- Read10X(&quot;1_Guided_tutorial/pbmc3k_filtered_gene_bc_matrices.tar.gz&quot;)</p>
  </blockquote>
  <p>Error in Read10X(&quot;1_Guided_tutorial/pbmc3k_filtered_gene_bc_matrices.tar.gz&quot;) :
  Directory provided does not exist</p>
  <p>I have set my working directory as the image showed. Can anyone let me know what is the problem? Thank you!</p>
  <p><a href="https://i.stack.imgur.com/hIQkx.png" rel="nofollow noreferrer"><img src="https://i.stack.imgur.com/hIQkx.png" alt="enter image description here" /></a></p>
  <pre><code>if (!requireNamespace(&quot;BiocManager&quot;, quietly = TRUE))
    install.packages(&quot;BiocManager&quot;)
  
  BiocManager::install(&quot;multtest&quot;)
  
  library(dplyr)
  library(Seurat)
  library(patchwork)
  
  # Load the PBMC dataset
  pbmc.data &lt;- Read10X(data.dir = &quot;1_Guided_tutorial/pbmc3k_filtered_gene_bc_matrices.tar.gz&quot;)
  </code></pre>