Many cases will also include a special standoff or post that lines up with the center mounting hole of the motherboard (provided it's ATX).
Many cases will also include a special standoff or post that lines up with the center mounting hole of the motherboard (provided it's ATX). This is very helpful in lining up the board inside the case. Once the board is inserted in the case with the post sticking through this hole, most of the rest of the screw holes should line up (or nearly line up) with the motherboard's standoffs.