question: How is active neurotoxin of Clostridium botulinum detected?
answer: Active neurotoxin of Clostridium botulinum can be detected by:
  mouse lethality assay
  by mass spectrometry
  bioassay
  differentiated cell models
  peptide cleavage assay
  FDC (functional dual coating) microtitre plate immuno-biochemical assay
  endopeptidase activity monitored via UV-Visible spectroscopy