dna polymerase assembles new strands in what direction
DNA replication DNA polymerase has 5'-3' activity. All known DNA replication systems require a free 3' hydroxyl group before synthesis can be initiated (note: the DNA template is read in 3' to 5' direction whereas a new strand is synthesized in the 5' to 3' directionâ€”this is often confused). Four distinct mechanisms for DNA synthesis are recognized: