Because such insertions are highly destabilizing, the formation of π-helices would tend to be selected against unless it provided some functional advantage to the protein. π-helices therefore are typically found near functional sites of proteins.
Pošto su takva umetanja veoma destabilišuća, do formiranja π-heliksa obično ne dolazi, osim ako to pruža funkcionalnu prednost proteinu. π-heliksi su stoga tipično prisutni u blizini funkcionalnih mesta proteina.