Upon conducting experiments following a modified Hanahan protocol using calcium chloride in E. coli, it was determined that spheroplasts may be able to transform at 4.9x10−4.
Facendo experimentos que seguían o protocolo de Hanahan modificado usando cloruro de calcio en E. coli, determinouse que os esferoplastos poden transformar cunha eficiencia de 4,9x10−4.