Some molecules or particles are too large or too hydrophilic to pass through a lipid bilayer even under these conditions, but can be moved across the membrane through fusion or budding of vesicles, events which have also been observed for freeze-thaw cycles.
Algunhas moléculas ou partículas son demasiado grandes ou excesivamente hidrófilas para pasar a través da bicapa lipídica incluso nesas condicións, pero poden moverse a través da membrana por fusión ou evaxinación de vesículas, events which have also been observed for freeze-thaw cycles.