Because the quantification of the results are analyzed by comparing the linear range of the target and control amplification, it is crucial to take into consideration the starting target molecules concentration and their amplification rate prior to starting the analysis.
Como a cuantificación dos resultados se analiza comparando o rango lineal da diana e a amplificación control, é crucial ter en conta a concentración das moéculas diana iniciais e a súa taxa de amplificación antes de empezar a análise.