Leptospira are typically cultivated at 30 °C in Ellinghausen-McCullough-Johnson-Harris (EMJH) medium, which can be supplemented with 0.21% rabbit serum to enhance growth of fastidious strains.
As Leptospira cultívanse tipicamente a 30 °C en medio Ellinghausen-McCullough-Johnson-Harris (EMJH), que pode ser suplementado con 0,2-1% de soro de coello para favorecer o crecemento de cepas moi esixentes.