Charles Sibley and Jon Ahlquist, pioneers of the technique, used DNA–DNA hybridization to examine the phylogenetic relationships of avians (the Sibley–Ahlquist taxonomy) and primates.
Charles Sibley e Jon Ahlquist foron os pioneiros desta técnica, e utilizaron a hibridación ADN–ADN para examinar as relacións filoxenéticas das aves (taxonomía das aves de Sibley–Ahlquist) e primates.