The next 40 to 50 cycles are the amplification program, which consists of three steps: (1) denaturation, (2) annealing, (3) elongation.
Os seguintes 40 a 50 ciclos constitúen o programa de amplificación, que consta de tres pasos: (1) desnaturatlización, (2) aliñamento (annealing), (3) elongación.