Chemical analysis in the 1980s showed that the junction actually preferred the antiparallel conformation, a finding that was considered controversial, and Robin Holliday himself initially doubted the findings.
Pero as análises químicas feitas na década de 1980 mostraron que, en realidade, a unión prefería as conformacións antiparalelas, o que foi un descubrimento considerado controvertido, e o propio Robin Holliday dubidaba inicialmente del.