In this method the proteins are first separated during the first dimension electrophoresis, then instead of the diffusion towards the antibodies, the proteins are electrophoresed into an antibody-containing gel in the second dimension.
Neste método as proteínas son primeiramente separadas durante a electroforese de primeira dimensión, despois en vez da difusión cara aos anticorpos, as proteínas son sometidas a electroforese nun xel que contén anticorpos na segunda dimensión.