The arterial supply to the pharyngotympanic tube is from several sources. Branches arise from the ascending pharyngeal artery (a branch of the external carotid artery) and from two branches of the maxillary artery (the middle meningeal artery and the artery of the pterygoid canal).
Venous drainage of the pharyngotympanic tube is to the pterygoid plexus of veins in the infratemporal fossa.