The recto-uterine pouch (pouch of Douglas) is an extremely important clinical region situated between the rectum and uterus. When the patient is in the supine position, the recto-uterine pouch is the lowest portion of the abdominopelvic cavity and is a site where infection and fluids typically collect. It is impossible to palpate this region transabdominally, but it can be examined by transvaginal and transrectal digital palpation. If an abscess is suspected, it may be drained through the vagina or the rectum without necessitating transabdominal surgery.