user389: Does anyone have any TOPO directional cloning tips? This question is board and essentially asking how to clone. However, it is practical and answerable but may be falling into a discussion. I was curious how to constraint what is a very well meaning question?

user798: I agree at the closure of this question within the format of stack ex, however to say that it will solely have answers backed by opinion I believe is incorrect. 
Example answer:

I find that using Pfu polymerase gave a particularly good yield of
  blunt ended PCR product which should be used in downstream TOPO
  cloning. [See agarose gel of PCR product]. I also tried NEB Phusion
  polymerase with drastically inferior yields [see agarose gel of poor
  yield]. So my tips is to use Pfu Polymerase.

I appreciate this question could be phrased as "which polymerase is best suited to directional TOPO cloning?" however I wanted to invite a broad range of insights before I begin the experiment so I am fully aware of problems or points of care that I should be aware of before I begin.

389: <p><a href="https://biology.stackexchange.com/q/2028/389">Does anyone have any TOPO directional cloning tips?</a> This question is board and essentially asking how to clone. However, it is practical and answerable but may be falling into a discussion. I was curious how to constraint what is a very well meaning question?</p>
 798: <p>I agree at the closure of this question within the format of stack ex, however to say that it will solely have answers backed by opinion I believe is incorrect. </p>

<p>Example answer:</p>

<blockquote>
  <p>I find that using Pfu polymerase gave a particularly good yield of
  blunt ended PCR product which should be used in downstream TOPO
  cloning. [See agarose gel of PCR product]. I also tried NEB Phusion
  polymerase with drastically inferior yields [see agarose gel of poor
  yield]. So my tips is to use Pfu Polymerase.</p>
</blockquote>

<p>I appreciate this question could be phrased as "which polymerase is best suited to directional TOPO cloning?" however I wanted to invite a broad range of insights before I begin the experiment so I am fully aware of problems or points of care that I should be aware of before I begin.</p>
