Multiparametric analysis of focal adhesion formation by RNAi-mediated gene knockdown

submitted by: JCB
Cell adhesion to the extracellular matrix is mediated by elaborate networks of multiprotein complexes consisting of adhesion receptors, cytoskeletal components, signaling molecules, and diverse adaptor proteins. To explore how specific molecular pathways function in the assembly of focal adhesions (FAs), we performed a high-throughput, high-resolution, microscopy-based screen. We used small interfering RNAs (siRNAs) to target human kinases, phosphatases, and migration- and adhesion-related...
Authors: Sabina Winograd-katz, Shalev Itzkovitz, Zvi Kam, Benjamin Geiger