demo/amd/rjs/output/unified/marionette-build.js
function(options) {
      options = options || {};
  
      this._firstRender = true;
      this._initializeRegions(options);
  
      Marionette.ItemView.call(this, options);
    }
function(options) {
      options = options || {};
  
      this._firstRender = true;
      this._initializeRegions(options);
  
      Marionette.ItemView.call(this, options);
    }
Ensure the regions are available when the `initialize` method is called.