def main(argv=None):
    if argv is None:
        argv = sys.argv

    parser = E.OptionParser(
        version="%prog version: $Id$",
        usage=globals()["__doc__"])

    parser.add_option("-g", "--genome-file", dest="genome_file", type="string",
                      help="filename with genomic sequence to retrieve "
                      "sequences from.")

    parser.add_option("-m", "--masker", dest="masker", type="choice",
                      choices=("dust", "dustmasker", "softmask", "none"),
                      help="apply masker to mask output sequences "
                      "[%default].")

    parser.add_option("--output-mode", dest="output_mode", type="choice",
                      choices=("intervals", "leftright", "segments"),
                      help="what to output. "
                      "'intervals' generates a single sequence for "
                      "each bed interval. 'leftright' generates two "
                      "sequences, one in each direction, for each bed "
                      "interval. 'segments' can be used to output "
                      "sequence from bed12 files so that sequence only covers "
                      "the segements [%default]")

    parser.add_option("--min-sequence-length", dest="min_length", type="int",
                      help="require a minimum sequence length [%default]")

    parser.add_option("--max-sequence-length", dest="max_length", type="int",
                      help="require a maximum sequence length [%default]")

    parser.add_option(
        "--extend-at", dest="extend_at", type="choice",
        choices=("none", "3", "5", "both", "3only", "5only"),
        help="extend at 3', 5' or both or no ends. If 3only or 5only "
        "are set, only the added sequence is returned [default=%default]")

    parser.add_option(
        "--extend-by", dest="extend_by", type="int",
        help="extend by # bases [default=%default]")

    parser.add_option(
        "--use-strand", dest="ignore_strand",
        action="store_false",
        help="use strand information and return reverse complement "
        "on intervals located on the negative strand. "
        "[default=%default]")

    parser.set_defaults(
        genome_file=None,
        masker=None,
        output_mode="intervals",
        min_length=0,
        max_length=0,
        extend_at=None,
        extend_by=100,
        ignore_strand=True,
    )

    (options, args) = E.Start(parser)

    if options.genome_file:
        fasta = IndexedFasta.IndexedFasta(options.genome_file)
        contigs = fasta.getContigSizes()
        fasta.setConverter(IndexedFasta.getConverter("zero-both-open"))

    counter = E.Counter()
    ids, seqs = [], []

    E.info("collecting sequences")
    for bed in Bed.setName(Bed.iterator(options.stdin)):
        counter.input += 1

        lcontig = fasta.getLength(bed.contig)

        if options.ignore_strand:
            strand = "+"
        else:
            strand = bed.strand

        if options.output_mode == "segments" and bed.columns == 12:
            ids.append("%s %s:%i..%i (%s) %s %s" %
                       (bed.name, bed.contig, bed.start, bed.end, strand,
                        bed["blockSizes"], bed["blockStarts"]))
            seg_seqs = [fasta.getSequence(bed.contig, strand, start, end)
                        for start, end in bed.toIntervals()]
            seqs.append("".join(seg_seqs))

        elif (options.output_mode == "intervals" or
              options.output_mode == "segments"):
            ids.append("%s %s:%i..%i (%s)" %
                       (bed.name, bed.contig, bed.start, bed.end, strand))
            seqs.append(
                fasta.getSequence(bed.contig, strand, bed.start, bed.end))

        elif options.output_mode == "leftright":
            l = bed.end - bed.start

            start, end = max(0, bed.start - l), l - bed.end
            ids.append("%s_l %s:%i..%i (%s)" %
                       (bed.name, bed.contig, start, end, strand))
            seqs.append(fasta.getSequence(bed.contig, strand, start, end))

            start, end = bed.start + l, min(lcontig, bed.end + l)
            ids.append("%s_r %s:%i..%i (%s)" %
                       (bed.name, bed.contig, start, end, strand))
            seqs.append(fasta.getSequence(bed.contig, strand, start, end))

    E.info("collected %i sequences" % len(seqs))

    masked = Masker.maskSequences(seqs, options.masker)
    options.stdout.write(
        "\n".join([">%s\n%s" % (x, y) for x, y in zip(ids, masked)]) + "\n")

    E.info("masked %i sequences" % len(seqs))

    counter.output = len(seqs)

    E.info("%s" % counter)

    E.Stop()
dataset/ETHPy150Open CGATOxford/cgat/scripts/bed2fasta.py/main/swapped operands of `-`