license:apache-2.0 region:us
---
license: apache-2.0
---

We downloaded a total of 2525 ClinVar datasets from the official ProteinGym. Since the official did not provide the corresponding structure files, we performed the following operations to obtain the structures:

1. According to the sequence, we retrieved the corresponding UniProt ID from the UniProt website and downloaded 2302 corresponding AlphaFold2 structures.
2. For the remaining proteins, we first folded them using ColabFold 1.5.5.
3. Among them, 12 proteins were too long to fold, and we solved this problem using the AlphaFold3 server.

## 📑 Github

https://github.com/tyang816/ProtSSN

## 🙌 Citation

Please cite our work if you have used our code or data for **dry experiment testing/wet experiment directed evolution**. We are pleased to see improvements in the subsequent work.

```
@article{tan2023protssn,
  title={Semantical and Topological Protein Encoding Toward Enhanced Bioactivity and Thermostability},
  author={Tan, Yang and Zhou, Bingxin and Zheng, Lirong and Fan, Guisheng and Hong, Liang},
  journal={bioRxiv},
  pages={2023--12},
  year={2023},
  publisher={Cold Spring Harbor Laboratory}
}