 BGlu0 also had high transglucosylation activity towards pyridoxine , producing pyridoxine 0-O-beta-D-glucopyranoside in the presence of the glucose donor p-nitrophenyl beta-D-glucoside . Score: 0.00 Title: Structural insights into rice BGlu0 beta-glucosidase oligosaccharide hydrolysis and transglycosylation . Journal: J Mol Biol Year: 0000 Type: MEDLINE Literature: oryza Field: abstract Doc ID: pub00000000 Accession (PMID): 00000000 Abstract: The structures of rice BGlu0 beta-glucosidase , a plant beta-glucosidase active in hydrolyzing cell wall-derived oligosaccharides , and its covalent intermediate with 0-deoxy-0-fluoroglucoside have been solved at 0 . 0 A and 0 . 00 A resolution , respectively . The structures were similar to the known structures of other glycosyl hydrolase family 0 ( GH0 ) beta-glucosidases , but showed several differences in the loops around the active site , which lead to an open active site with a narrow slot at the bottom , compatible with the hydrolysis of long beta-0 , 0-linked oligosaccharides . Though this active site structure is somewhat similar to that of the Paenibacillus polymyxa beta-glucosidase B , which hydrolyzes similar oligosaccharides , molecular docking studies indicate that the residues interacting with the substrate beyond the conserved -0 site are completely different , reflecting the independent evolution of plant and microbial GH0 exo-beta-glucanase/beta-glucosidases . The complex with the 0-fluoroglucoside included a glycerol molecule , which appears to be in a position to make a nucleophilic attack on the anomeric carbon in a transglycosylation reaction . The coordination of the hydroxyl groups suggests that sugars are positioned as acceptors for transglycosylation by their interactions with E000 , the catalytic acid/base , and Y000 , which is conserved in barley BGQ00/beta-II beta-glucosidase , that has oligosaccharide hydrolysis and transglycosylation activity similar to rice BGlu0 . As the rice and barley enzymes have different preferences for cellobiose and cellotriose , residues that appeared to interact with docked oligosaccharides were mutated to those of the barley enzyme to see if the relative activities of rice BGlu0 toward these substrates could be changed to those of BGQ00 . Although no single residue appeared to be responsible for these differences , I000 , N000 and N000 did appear to interact with the substrates . Matching Sentences: [ Sen. 0, subscore: 0.00 ]: The structures of rice BGlu0 beta-glucosidase , a plant beta-glucosidase active in hydrolyzing cell wall-derived oligosaccharides , and its covalent intermediate with 0-deoxy-0-fluoroglucoside have been solved at 0 . 0 A and 0 . 00 A resolution , respectively . [ Sen. 0, subscore: 0.00 ]: The coordination of the hydroxyl groups suggests that sugars are positioned as acceptors for transglycosylation by their interactions with E000 , the catalytic acid/base , and Y000 , which is conserved in barley BGQ00/beta-II beta-glucosidase , that has oligosaccharide hydrolysis and transglycosylation activity similar to rice BGlu0 . 