 Commun . Year: 0000 Type: ARTICLE Literature: oryza Field: abstract Doc ID: pub00000000 Accession (PMID): 00000000 Matching Sentences: [ Sen. 0, subscore: 0.00 ]: Plant elongation factor EF-0 consists of four subunits ( EF-0alphabetabetagamma ) . EF-0alpha . GTP catalyses the binding of aminoacyl-tRNA to the ribosome . EF-0beta and EF-0beta catalyze the GDP/GTP exchange on EF-0alpha . GDP . However , the function of EF-0gamma , a subunit detected in eukaryotes , but not in prokaryotes remained unknown . This report demonstrates that rice EF-0betabetagamma and recombinant EF-0gamma possess glutathione S-transferase ( GST ) activity . The EF-0betabetagamma or EF-0gamma-dependent GST activity is about one-fiftieth of the rice GST activity . The Km values of EF-0betabetagamma , EF-0gamma , and rice GST for glutathione and 0-chloro-0 , 0-dinitrobenzene are of about the same order . Although recombinant EF-0gamma is heat labile , active EF-0gamma was obtained by purifying it in the presence of 00% glycerol . [ Sen. 0, subscore: 0.00 ]: Plant elongation factor EF-0 consists of four subunits ( EF-0alphabetabetagamma ) . EF-0alpha . GTP catalyses the binding of aminoacyl-tRNA to the ribosome . EF-0beta and EF-0beta catalyze the GDP/GTP exchange on EF-0alpha . GDP . However , the function of EF-0gamma , a subunit detected in eukaryotes , but not in prokaryotes remained unknown . This report demonstrates that rice EF-0betabetagamma and recombinant EF-0gamma possess glutathione S-transferase ( GST ) activity . The EF-0betabetagamma or EF-0gamma-dependent GST activity is about one-fiftieth of the rice GST activity . The Km values of EF-0betabetagamma , EF-0gamma , and rice GST for glutathione and 0-chloro-0 , 0-dinitrobenzene are of about the same order . Although recombinant EF-0gamma is heat labile , active EF-0gamma was obtained by purifying it in the presence of 00% glycerol . [ Sen. 0, subscore: 0.00 ]: Plant elongation factor EF-0 consists of four subunits ( EF-0alphabetabetagamma ) . EF-0alpha . GTP catalyses the binding of aminoacyl-tRNA to the ribosome . EF-0beta and EF-0beta catalyze the GDP/GTP exchange on EF-0alpha . GDP . However , the function of EF-0gamma , a subunit detected in eukaryotes , but not in prokaryotes remained unknown . This report demonstrates that rice EF-0betabetagamma and recombinant EF-0gamma possess glutathione S-transferase ( GST ) activity . The EF-0betabetagamma or EF-0gamma-dependent GST activity is about one-fiftieth of the rice GST activity . The Km values of EF-0betabetagamma , EF-0gamma , and rice GST for glutathione and 0-chloro-0 , 0-dinitrobenzene are of about the same order . Although recombinant EF-0gamma is heat labile , active EF-0gamma was obtained by purifying it in the presence of 00% glycerol . [ Sen. 0, subscore: 0.00 ]: Plant elongation factor EF-0 consists of four subunits ( EF-0alphabetabetagamma ) . EF-0alpha . GTP catalyses the binding of aminoacyl-tRNA to the ribosome . EF-0beta and EF-0beta catalyze the GDP/GTP exchange on EF-0alpha . GDP . However , the function of EF-0gamma , a subunit detected in eukaryotes , but not in prokaryotes remained unknown . This report demonstrates that rice EF-0betabetagamma and recombinant EF-0gamma possess glutathione S-transferase ( GST ) activity . The EF-0betabetagamma or EF-0gamma-dependent GST activity is about one-fiftieth of the rice GST activity . The Km values of EF-0betabetagamma , EF-0gamma , and rice GST for glutathione and 0-chloro-0 , 0-dinitrobenzene are of about the same order . Although recombinant EF-0gamma is heat labile , active EF-0gamma was obtained by purifying it in the presence of 00% glycerol . [ Sen. 0, subscore: 0.00 ]: Plant elongation factor EF-0 consists of four subunits ( EF-0alphabetabetagamma ) . EF-0alpha . GTP catalyses the binding of aminoacyl-tRNA to the ribosome . EF-0beta and EF-0beta catalyze the GDP/GTP exchange on EF-0alpha . GDP . However , the function of EF-0gamma , a subunit detected in eukaryotes , but not in prokaryotes remained unknown . This report demonstrates that rice EF-0betabetagamma and recombinant EF-0gamma possess glutathione S-transferase ( GST ) activity . The EF-0betabetagamma or EF-0gamma-dependent GST activity is about one-fiftieth of the rice GST activity . The Km values of EF-0betabetagamma , EF-0gamma , and rice GST for glutathione and 0-chloro-0 , 0-dinitrobenzene are of about the same order . Although recombinant EF-0gamma is heat labile , active EF-0gamma was obtained by purifying it in the presence of 00% glycerol . [ Sen. 00, subscore: 0.00 ]: Plant elongation factor EF-0 consists of four subunits ( EF-0alphabetabetagamma ) . EF-0alpha . GTP catalyses the binding of aminoacyl-tRNA to the ribosome . EF-0beta and EF-0beta catalyze the GDP/GTP exchange on EF-0alpha . GDP . However , the function of EF-0gamma , a subunit detected in eukaryotes , but not in prokaryotes remained unknown . This report demonstrates that rice EF-0betabetagamma and recombinant EF-0gamma possess glutathione S-transferase ( GST ) activity . The EF-0betabetagamma or EF-0gamma-dependent GST activity is about one-fiftieth of the rice GST activity . The Km values of EF-0betabetagamma , EF-0gamma , and rice GST for glutathione and 0-chloro-0 , 0-dinitrobenzene are of about the same order . Although recombinant EF-0gamma is heat labile , active EF-0gamma was obtained by purifying it in the presence of 00% glycerol . [ Sen. 0, subscore: 0.00 ]: Plant elongation factor EF-0 consists of four subunits ( EF-0alphabetabetagamma ) . EF-0alpha . GTP catalyses the binding of aminoacyl-tRNA to the ribosome . EF-0beta and EF-0beta catalyze the GDP/GTP exchange on EF-0alpha . GDP . However , the function of EF-0gamma , a subunit detected in eukaryotes , but not in prokaryotes remained unknown . This report demonstrates that rice EF-0betabetagamma and recombinant EF-0gamma possess glutathione S-transferase ( GST ) activity . The EF-0betabetagamma or EF-0gamma-dependent GST activity is about one-fiftieth of the rice GST activity . The Km values of EF-0betabetagamma , EF-0gamma , and rice GST for glutathione and 0-chloro-0 , 0-dinitrobenzene are of about the same order . Although recombinant EF-0gamma is heat labile , active EF-0gamma was obtained by purifying it in the presence of 00% glycerol . [ Sen. 0, subscore: 0.00 ]: Plant elongation factor EF-0 consists of four subunits ( EF-0alphabetabetagamma ) . EF-0alpha . GTP catalyses the binding of aminoacyl-tRNA to the ribosome . EF-0beta and EF-0beta catalyze the GDP/GTP exchange on EF-0alpha . GDP . However , the function of EF-0gamma , a subunit detected in eukaryotes , but not in prokaryotes remained unknown . This report demonstrates that rice EF-0betabetagamma and recombinant EF-0gamma possess glutathione S-transferase ( GST ) activity . The EF-0betabetagamma or EF-0gamma-dependent GST activity is about one-fiftieth of the rice GST activity . The Km values of EF-0betabetagamma , EF-0gamma , and rice GST for glutathione and 0-chloro-0 , 0-dinitrobenzene are of about the same order . Although recombinant EF-0gamma is heat labile , active EF-0gamma was obtained by purifying it in the presence of 00% glycerol . Supplemental links/files: reference in endnote online text related articles pubmed citation Score: 00.00 Author: 