 Map-based cloning revealed that the bgl locus encodes OsRopGEF00 , one of eleven OsRopGEFs in rice . Score: 0.00 Title: The role of the oligosaccharide binding cleft of rice BGlu0 in hydrolysis of cellooligosaccharides and in their synthesis by rice BGlu0 glycosynthase . Journal: Protein Sci Year: 0000 Type: MEDLINE Literature: oryza Field: abstract Doc ID: pub00000000 Accession (PMID): 00000000 Abstract: Rice BGlu0 beta-glucosidase nucleophile mutant E000G is a glycosynthase that can synthesize p-nitrophenyl ( pNP ) -cellooligosaccharides of up to 00 residues . The X-ray crystal structures of the E000G glycosynthase with and without alpha-glucosyl fluoride were solved and the alpha-glucosyl fluoride complex was found to contain an ordered water molecule near the position of the nucleophile of the BGlu0 native structure , which is likely to stabilize the departing fluoride . The structures of E000G glycosynthase in complexes with cellotetraose and cellopentaose confirmed that the side chains of N000 , S000 , and Y000 interact with glucosyl residues in cellooligosaccharide binding subsites +0 , +0 , and +0 . Mutants in which these residues were replaced in BGlu0 beta-glucosidase hydrolyzed cellotetraose and cellopentaose with k ( cat ) /K ( m ) values similar to those of the wild type enzyme . However , the Y000A , Y000L , and N000V mutants of the E000G glycosynthase synthesize shorter pNP-cellooligosaccharides than do the E000G glycosynthase and its S000A mutant , suggesting that Y000 and N000 play important roles in the synthesis of long oligosaccharides . X-ray structural studies revealed that cellotetraose binds to the Y000A mutant of the glycosynthase in a very different , alternative mode not seen in complexes with the E000G glycosynthase , possibly explaining the similar hydrolysis , but poorer synthesis of longer oligosaccharides by Y000 mutants . Matching Sentences: [ Sen. 0, subscore: 0.00 ]: Rice BGlu0 beta-glucosidase nucleophile mutant E000G is a glycosynthase that can synthesize p-nitrophenyl ( pNP ) -cellooligosaccharides of up to 00 residues . 