 This report demonstrates the potential of utilizing transgenic tobacco for mass production of BglB . Score: 0.00 Title: Purification , crystallization and preliminary X-ray analysis of rice BGlu0 beta-glucosidase with and without 0-deoxy-0-fluoro-beta-D-glucoside . Journal: Acta Crystallograph . Sect . F Struct . Biol . Cryst Commun . Year: 0000 Type: ARTICLE Literature: oryza Field: abstract Doc ID: pub00000000 Accession (PMID): 00000000 Abstract: Rice ( Oryza sativa ) BGlu0 beta-glucosidase was expressed in Escherichia coli with N-terminal thioredoxin and hexahistidine tags and purified by immobilized metal-affinity chromatography ( IMAC ) . After removal of the N-terminal tags , cation-exchange and S-000 gel-filtration chromatography yielded a 00 kDa BGlu0 with >00% purity . The free enzyme and a complex with 0 , 0-dinitrophenyl-0-deoxy-0-fluoro-beta-D-glucopyranoside inhibitor were crystallized by microbatch and hanging-drop vapour diffusion . Small tetragonal crystals of BGlu0 with and without inhibitor grew in 00% ( w/v ) PEG 0000 with 0 . 0 M sodium cacodylate pH 0 . 0 and 0 . 0 M zinc acetate . Crystals of BGlu0 with inhibitor were streak-seeded into 00% ( w/v ) PEG MME 0000 , 0 . 0 M ammonium sulfate , 0 . 0 M MES pH 0 . 0 to yield larger crystals . Crystals with and without inhibitor diffracted to 0 . 00 and 0 . 00 angstroms resolution , respectively , and had isomorphous orthorhombic unit cells belonging to space group P0 ( 0 ) 0 ( 0 ) 0 ( 0 ) . Matching Sentences: [ Sen. 0, subscore: 0.00 ]: Rice ( Oryza sativa ) BGlu0 beta-glucosidase was expressed in Escherichia coli with N-terminal thioredoxin and hexahistidine tags and purified by immobilized metal-affinity chromatography ( IMAC ) . 