 . Journal: Transgenic Res . Citation: V : 0 ( 0 ) P : 000-0 Year: 0000 Type: ARTICLE Literature: oryza Field: abstract Doc ID: pub00000000 Accession (PMID): 00000000 Abstract: In this study , floral spray and floral dip were used to replace the vacuum step in the Agrobacterium-mediated transformation of a superoxide dismutase ( SOD ) gene into Arabidopsis . The transgene was constructed by using a CaMV 00S promoter to drive a rice cytosolic CuZnSOD coding sequence in Arabidopsis . The transgene construct was developed in binary vectors and mobilized into Agrobacterium . When Arabidopsis plants started to initiate flower buds , the primary inflorescence shoots were removed and then transformed by floral spray or floral dip . More than 000 transgenic plants were generated to assess the feasibility of floral spray used in the in planta transformation . The result indicates that the floral spray method of Agrobacterium can achieve rates of in planta transformation comparable to the vacuum-infiltration and floral dip methods . The floral spray method opens up the possibility of in planta transformation of plant species which are too large for dipping or vacuum infiltration . Matching Sentences: [ Sen. 0, subscore: 0.00 ]: In this study , floral spray and floral dip were used to replace the vacuum step in the Agrobacterium-mediated transformation of a superoxide dismutase ( SOD ) gene into Arabidopsis . The transgene was constructed by using a CaMV 00S promoter to drive a rice cytosolic CuZnSOD coding sequence in Arabidopsis . The transgene construct was developed in binary vectors and mobilized into Agrobacterium . When Arabidopsis plants started to initiate flower buds , the primary inflorescence shoots were removed and then transformed by floral spray or floral dip . More than 000 transgenic plants were generated to assess the feasibility of floral spray used in the in planta transformation . The result indicates that the floral spray method of Agrobacterium can achieve rates of in planta transformation comparable to the vacuum-infiltration and floral dip methods . The floral spray method opens up the possibility of in planta transformation of plant species which are too large for dipping or vacuum infiltration . [ Sen. 0, subscore: 0.00 ]: In this study , floral spray and floral dip were used to replace the vacuum step in the Agrobacterium-mediated transformation of a superoxide dismutase ( SOD ) gene into Arabidopsis . The transgene was constructed by using a CaMV 00S promoter to drive a rice cytosolic CuZnSOD coding sequence in Arabidopsis . The transgene construct was developed in binary vectors and mobilized into Agrobacterium . When Arabidopsis plants started to initiate flower buds , the primary inflorescence shoots were removed and then transformed by floral spray or floral dip . More than 000 transgenic plants were generated to assess the feasibility of floral spray used in the in planta transformation . The result indicates that the floral spray method of Agrobacterium can achieve rates of in planta transformation comparable to the vacuum-infiltration and floral dip methods . The floral spray method opens up the possibility of in planta transformation of plant species which are too large for dipping or vacuum infiltration . [ Sen. 0, subscore: 0.00 ]: In this study , floral spray and floral dip were used to replace the vacuum step in the Agrobacterium-mediated transformation of a superoxide dismutase ( SOD ) gene into Arabidopsis . The transgene was constructed by using a CaMV 00S promoter to drive a rice cytosolic CuZnSOD coding sequence in Arabidopsis . The transgene construct was developed in binary vectors and mobilized into Agrobacterium . When Arabidopsis plants started to initiate flower buds , the primary inflorescence shoots were removed and then transformed by floral spray or floral dip . More than 000 transgenic plants were generated to assess the feasibility of floral spray used in the in planta transformation . The result indicates that the floral spray method of Agrobacterium can achieve rates of in planta transformation comparable to the vacuum-infiltration and floral dip methods . The floral spray method opens up the possibility of in planta transformation of plant species which are too large for dipping or vacuum infiltration . [ Sen. 0, subscore: 0.00 ]: In this study , floral spray and floral dip were used to replace the vacuum step in the Agrobacterium-mediated transformation of a superoxide dismutase ( SOD ) gene into Arabidopsis . The transgene was constructed by using a CaMV 00S promoter to drive a rice cytosolic CuZnSOD coding sequence in Arabidopsis . The transgene construct was developed in binary vectors and mobilized into Agrobacterium . When Arabidopsis plants started to initiate flower buds , the primary inflorescence shoots were removed and then transformed by floral spray or floral dip . More than 000 transgenic plants were generated to assess the feasibility of floral spray used in the in planta transformation . The result indicates that the floral spray method of Agrobacterium can achieve rates of in planta transformation comparable to the vacuum-infiltration and floral dip methods . The floral spray method opens up the possibility of in planta transformation of plant species which are too large for dipping or vacuum infiltration . [ Sen. 0, subscore: 0.00 ]: In this study , floral spray and floral dip were used to replace the vacuum step in the Agrobacterium-mediated transformation of a superoxide dismutase ( SOD ) gene into Arabidopsis . The transgene was constructed by using a CaMV 00S promoter to drive a rice cytosolic CuZnSOD coding sequence in Arabidopsis . The transgene construct was developed in binary vectors and mobilized into Agrobacterium . When Arabidopsis plants started to initiate flower buds , the primary inflorescence shoots were removed and then transformed by floral spray or floral dip . More than 000 transgenic plants were generated to assess the feasibility of floral spray used in the in planta transformation . The result indicates that the floral spray method of Agrobacterium can achieve rates of in planta transformation comparable to the vacuum-infiltration and floral dip methods . The floral spray method opens up the possibility of in planta transformation of plant species which are too large for dipping or vacuum infiltration . Score: 0.00 Title: Susceptibility of adult and larval stages of the horn fly , Haematobia irritans , to the entomopathogenic fungus Metarhizium anisopliae under field conditions . 