echnol Year: 0000 Type: MEDLINE Literature: oryza Field: abstract Doc ID: pub00000000 Accession (PMID): 00000000 Abstract: A novel beta-glucosidase ( BGL ) -producing strain was isolated and identified as Penicillium purpurogenum KJS000 based on its morphology and internal transcribed spacer ( ITS ) rDNA gene sequence . When rice straw and corn steep powder were used as carbon and nitrogen sources , respectively , the maximal BGL activity of 00 . 0 U ml ( -0 ) , one of the highest levels among BGL-producing microorganisms was observed . The optimum temperature and pH for BGL production were 00 degrees C and 0 , respectively . An extracellular BGL was purified to homogeneity by sequential chromatography of P purpurogenum culture supernatants , and the purified BGL showed higher activity ( V ( max ) = 000 U mg protein ( -0 ) ) than most BGLs from other sources . The complete ORF of bgl0 was cloned from P purpurogenum by a modified thermal asymmetric interlaced polymerase chain reaction . The bgl0 gene consists of a 0 , 000-bp ORF and encodes a putative protein containing 000 amino acids with a calculated molecular mass of 00 , 000 Da . 