Unsaturated fatty acids are synthesized in the
Plasma membrane
Endoplasmic reticulum
Cytosol
Golgi complex
Most of the activity of these enzymes in the biosynthesis of triacylglycerols resides in the endoplasmic reticulum, but some are found in mitochondria.Ref: Harper&;s Biochemistry; 30th edition; Chapter 24; Metabolism of Acylglycerols & Sphingolipids
Metabolism of lipid