Isoelectric point of a protein is located by which method?
Isoelectric pointing
Isoelectric cardiac failure
Ion exchange
pH
Ans. is 'b' i.e.,Isoelectric cardiac failure Isoelectric focusing is a procedure used to determine the isoelectric point (pI) of a protein.A pH gradient is established by allowing a mixture of low molecular weight organic acids and bases to distribute themselves in an electric field generated across the gel.When a protein mixture is applied, each protein migrates until it reaches the pH that matches its pI.Proteins with different isoelectric points are thus distributed differently throughout the gel.