The activity of pyruvate carboxylase is dependent upon the positive allosteric effector
Succinate
AMP
Isocitrate
Acetyl Co A
Pyruvate carboxylase is an allosteric enzyme. Acetyl CoA is an activator of pyruvate carboxylase so the generation of oxaloacetate is ored when acetyl CoA level is sufficiently high.Ref: DM Vasudevan, 7th edition, page no: 120
Metabolism of carbohydrate