True statement regarding Hirschsprung's disease –
Giant ganglia are present
Mucosa is involved and show foldings
Manometry excludes the disease
Rectal biopsy is contraindicated in infants
Anorectal manometry is a useful screening procedure for the diagnosis of Hirschsprung's disease. A normal response in course of manometric evaluation precludes diagnosis of Hirschsprung's disease.
However the confirmation of diagnosis in case of Hirschsprung's disease is done by rectal biopsy.