Gram-negative bacteria doesn't take gram stain because it is made of
Polysaccharide
Lipopolysaccharide
Techoic acid
None of the above
The exact mechanism of gram reaction is not known. The possible reason is: Gram-positive bacteria have a more acidic protoplasm, which accounts for their retaining the basic primary dye more strongly. Dye iodine complex is retained in gram-positive cells by thick peptide glycan mesh and is readily wash through the very thin peptidoglycan layer of gram-negative cells. Ref: Ananthanarayana textbook of Microbiology 9th edition Pgno : 13
All India exam