Which of the following fungi is/are difficult to isolate culture ?
Candida
Dermatophytes
Cryptococcus
Malassezia furfur
Ans. is 'A' i.e., Malassezia furfur Malassezia furfur does not grow on regular sabouraud's medium. It requires complex media to grow M. furfur is a lipid dependent fungus and 1% emulsified olive oil is added to sabouraud medium for its cultivation Two media are now widely employed for all malassezia species. Dixon medium Modified dixon medium