Which one of the following factors released by heating a suspension of sheep erythrocytes is required for the growth of Haemophilus Influenzae in chocolate agar?
Hemin
Nicotinamide adenine dinucleotide (NAD)
Hemoglobin
Hemolysin
Ans. B. NAD.a. Members of Hemophilus require for one or more of the accessory factors: X & V in bloodb. Factor X is heme whereas factor V is NAD/NADP.c. The V factor (NAD) is trapped in the RBC's in blood agar. Hence, on blood agar, Hemophilus influenzae shows poor growth.d. By heating blood agar, V factor is released into the medium.
Bacteria