An immunologist is working on growing influenza virus for vaccine production in national institute of virology, Bangalore. Which of the following sites is used for the production of vaccine in a feilized egg?
Allantoic cavity
chorioallontoic membrane
Yolk sac
Amniotic cavity
Allantoic cavity inoculation is used for the growth and replication of the influenza virus for vaccine production. Embryonated eggs are used for the cultivation of viruses. Virus cultivation- Animal, Embryonated Egg and tissue culture Animal inocultion- suckling mice is used for inoculation of coxsackie and arbovirus Embryonated egg inoculation- Chorioallantoic membrane- Vaccinia, Variola, HSV 1 and HSV 2 Allantoic cavity- Vaccine of Yellow fever (17D), Influenza, Rabies Amniotic sac- Isolation of influenza Yolk sac- Chlamydiae, Rickettsiae Tissue culture- Cell lines Primary cell lines- Rhesus kidney cell line, Human amniotic cell line, Chick embyro fibroblast Secondary cell lines- Human fibroblast cell line, MRC-5 and WI- 38 Continuous cell lines- HeLa cell line, HEp-2, KB, Vero Reference: Ananthanarayan and Paniker&;s Textbook of Microbiology Tenth edition
Virology