What does the term nick translation refer to?
Translation of RNA into polypeptide
Regulation of gene expression
Technique to label DNA
Technique to digest DNA
A nick is a discontinuity in a double stranded DNA molecule where there is no phosphodiester bond. Nick is created by DNAse I Nick translation is done by DNA polymerase I Nick sealing is done by DNA ligase Nick translation removes DNA ahead of a nick, synthesizes DNA behind nick and as a net result, moves the nick in the 5' to 3' direction. This propey is used to incorporate radio labeled nucleotides in DNA.
Techniques in molecular biology