The rate of newly synthesized Osteoid mineralization is best estimated by
Tetracycline labelling
Alizarine red staining
Calceine stain
Von Kossa stain
Reference Robbins page no 358 6th edition Tetracycline is absorbed into bone and so it is used as a marker of bone growth for biopsies in humans. Tetracycline binds to newly formed bone at the bone interface where it shows as a linear fluroscencse. Tetracycline labelling is used to determine the amount of bone growth within a ceain period of time
Musculoskeletal system