All the following can be used to detect the mutation, except
Single strand conformational polymorphism
Ligase chain reaction
Polymerase chain reaction
DNA Sequencing
Methods used for detecting mutations 

Cytogenetics analysis  
FISH   
PCR, 
RFLP 
DNA sequencing, Southern blotting 
Microarrays 
Single-stranded conformational polymorphism (SSCP)
RNAase cleavage
Denaturating gradient gel electrophoresis (DGGE)
Protein truncation test