Stroma of cornea is developed from
Neural ectoderm
Surface ectoderm
Mesoderm
Neural crest
Stroma of cornea develops from neural crest cells derived (secondary) mesenchyme. The adult cornea has developmentally three layers: Outer epithelium layer (surface ectoderm) Middle stromal layer of collagen-rich extracellular matrix between stromal keratocytes (neural crest) Inner layer of endothelial cells (neural crest). Ref - medscape.com
General anatomy