Molecular separation of two proteins with same charge can be done by
Ion exchange chromatography
Dialysis
Gel diffusion chromatography
Electrophoresis
Molecular separation of two proteins with same charge can be done by gel diffusion chromatography. Gel diffusion chromatography is the best method to differentiate proteins. Ref: Harper 28th Ed. Page-21-22, Harper 27th Ed. Page: 21-22
Structure and function of protein