The following is not a feature of malignant transformation by culturedc
Increased cell density
Increased requirement for growth factors
Alterations of cytoskeleton structure
Loss of anchorage
Ref biology of the cell p357-364 Both normal cells and cancer cells can be cultured in-vitro , however they behave quite differently normal cell: show replicative senescence .is cells pass through limited number of cell division before they decline in vigor and die it may caused by inability to synthesise telomerase
General anatomy