Berson and yellow 1st described the following test
RIA
ELISA
Immuno chromatography
Chemiluminescence assay
Berson and Yalow discovered RIA in 1959 by which we can quantify antigens or hormones upto picograms-v sensitive than ELISA RIA /ELISA-indirect immunofluorescence tests Ref: Textbook of Microbiology Baveja 5th ed Pg 115
Immunology