In a patient with urethral syndrome, urine microscopy shows full of polymorph, but no bacteria. The most appropriate culture medium is__
PPLO broth
McCoy cells
Robeson's cooked meat
Thayer main agar
The clinical picture is suggestive of Chlamydial infection hence the most appropriate culture medium is McCoy cells(human synol carcinoma cell line). Abnormal urethral Gram's stains showing >10 PMNs/1000A~ field in women with dysuria but without coliform bacteriuria suppo the diagnosis of chlamydial urethritis. Thayer-Main agar: Neisseria Robeson's cooked meat: Anaerobes, especially Clostridia Pleuropneumonia-like organisms (PPLO) broth: Mycoplasma spp. and Ureaplasma spp. Reference: Ananthanarayan and Paniker&;s Textbook of Microbiology Tenth edition
Bacteriology