Which of the following technique is used to find out the impoant amino acid residue involved in the enzyme catalysis?
Electrophoresis
Chromatography
Mass spectrometry
Site directed mutagenesis
Codon for specific aminoacids can be altered using site-directed mutagenesis. Site-directed mutagenesis Identification of the specific roles of given aminoacyl residues in substrate binding and catalysis. For eg : A paicular aminoacyl residue functions as a general acid can be tested by replacing it with an aminoacyl residue incapable of donating a proton.
Techniques in molecular biology