Hybridoma used in:
In situ hybridization
Sequencing DNA
Production of monoclonal antibodies
Production of continuous cell lines
ln 1975, George Kohler and Cesar Milstein created hybrid cells that will make unlimited quantities of antibodies with defined specificities, which are termed as monoclonal antibodies . This discovery know as hybridoma technology Principle - fusion between myeloma cell (malignant plasma cell) and spleen cell from a immunized animal. Hybridoma technology can make available highly specific antibodies in abundant amounts. use of monoclonal antibodies Example of monoclonal ab trastuzumab - EGRF/HER2 INHIBITORS BEVACIZUMAB - ANGIOGENESIS INHIBITORS
DNB 2018