RELP, True are
Endonuclease cuts DNA at nucleotide level
It acts at specific site
Only cohesive ends are produced
Only blunt ends are produced
The basic technique for the detecting of RFLPs involves fragmenting a sample of DNA by a restriction enzyme, which can recognize and cut DNA wherever a specific sho sequence occurs, in a process known as a restriction digest. The resulting DNA fragments are then separated by length through a process known as agarose gel electrophoresis and transferred to a membrane the Southern blot procedure. Hybridization of the membrane to a labeled DNA probe then determines the length of the fragments which are complementary to the probe. An RFLP occurs when the length of a detected fragment varies between individuals. Each fragment length is considered an allele and can be used in genetic analysis.RFLP analysis may be subdivided into single- (SLP) and multi-locus probe (MLP) paradigms. Usually, the SLP method is preferred over MLP because it is more sensitive, easier to interpret and capable of analyzing mixed-DNA samples. Moreover, data can be generated even when the DNA is degraded (e.g. when it is found in bone remains.)Ref: https://en.wikipedia.org/wiki/Restriction_fragment_length_polymorphism
Metabolism of nucleic acids