Fixative used in PAP smear:
55% ethyl alcohol
95% formalin
95% ethyl alcohol
Normal saline
Pap smear Screening test Done by Ayre's spatula Sensitivity of pap smear is 47 to 62 % If cytobrush used - | sensitivity upto 90% Site Transformation zone (squamocolumnar junction) Endometrium - is columnar Vagina & cervix - is squamous Fixative used in pap Smear - 95 % ethyl alcohol. Technique To sample the ectocervix, a wooden spatula is placed against the external as and rotated 360 degrees. To sample the endocervix, a cytobrush is inseed into the external os and rotated. The sample obtained by the spatula and cytobrush is spread on a glass slide and immediately placed in a fixative, usually 95% ethanol, fixed for 30 minutes, and then stained with Papanicolaou stain. The sample is stained using the Papanicolaou technique in which tinctorial dyes and acids are selectively retained by cells.
Cervical Carcinoma