A 20 years old male patient presents to the STD clinic with a genital ulcer. The gram stain of the smear from ulcer shows gram negative coccobacilli. The most appropriate media for culture would be:
Thayer Main Medium
Blood agar with X & V factors
Chocolate agar with IsoVitaleX
Teilurite blood agar
The clinical picture is suggestive of soft sore (chancroid) caused by Haemophilus ducreyi. Chocolate agar with IsoVitaleX is used for its culture. Other culture media: Thayer Main Medium - Gonococci Blood agar with X & V factors - Haemophilus influenzae Tellurite blood agar - Corynbacterium diphtheriae Reference: Ananthanarayan and Paniker&;s Textbook of Microbiology Tenth edition
Bacteriology