Sections from a solid-cystic unilateral ovarian tumor in a 30-year old female show a tumor composed of diffuse sheets of small cells with doubtful nuclear grooving and scanty cytoplasm. No Call-Exner bodies are seen.The ideal immunohistochemistry panel would include -
Vimentin, epithelial membrane antigen, inhibin, CD99
Desmin, S-100 protein, smooth muscle antigen, cytokeratin
Chromogranin, CD45, CD99, CD20
CD3, chromagranin, CD 45, synaptophysin
Ans. is 'a' i.e., Vimentin, epithelial membrane antigen, inhibin, CD99This patient is having granulosa cell tumor of ovary which is chracterized histologically by :(i) Diffuse sheets of round and oval cells (ii) Inconspicuous groove and folds in nuclei - coffee bean appearance(iii) Scant cytoplasmo Granulosa cell tumor is positive for --> (i) Vimentin, (ii) Inhibin, (iii) CD99o Epithelial membrane antigen is positive in surface epithelial tumor.So, a option a is the best, and markers given in this option will help to make the diagnosis of granulosa cell tumor.