Rate of mineralization of newly formed osteoid can be estimated by the following:
Von Kossa staining for calcium
Alzarin red stain
Labelled tetracycline
Immunofluorescence
Tetracycline -becomes fixed in new forming mineralizing bone, exhibits a characteristic fluorescence when viewed by UV light 2 doses of tetracycline given a few of days apa, 2 bands of fluorescence- separated by an interval of unlabelled new bone formed during the period between doses. Rate of mineralization of newly formed osteoid is estimated by tetracycline labelling
Fracture Management