Chlamydia are isolated by
Enzyme immune assay
Yolk sac inoculation
Direct immunofluorescence
Microimmunofluorescence
Isolation of chlamydiae can be done by :Inoculation into yolk sac/embryonated eggs of 6-8 day old chick embryo which may be pretreated by streptomycin or polymyxin B.Inoculation into experimental animals (mice)Tissue/cell culture:Preferred modeCommonly used cell lines are McCoy and HeLa cells.Cell cultures are pretreated by irradiation or chemicals such as 5-iodo-2 deoxyuridines or cycloheximide to enhance replication and detection of inclusion bodies.Pretreatment with DEAE dextran or centrifugation after inoculation promotes contact between chlamydiae paicles and cell monolayer.
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