A large dose of EDTA is used in carbonic anhydrase enzyme inactivation. The mechanism by which EDTA acts is -
It chelates with the metal ion of the enzyme
It combines with the substrate and doesn't react with the enzyme
Combines with the substrate and reacts with the enzyme
Enzyme-EDTA complex can not be attached to substrate
Ans. is 'a' i.e., It chelates with ions of the enzyme EDTA is a chelating agent. It acts by causing chelation.Chelation is a chemical reaction that results in bond being formed between a metal ion and an organic (Le., carbon based) molecule.The resulting complex metal bound to molecule is called a "chelate" and contains one or more rings of atoms in which the metal ion is so firmly bound it cannot escape: The complex is stable nontoxic and is easily excreted.EDTA is a chelating agent. It is used to inactivate carbonic anhydrase enzyme.It does so by binding with the metal (zinc) ion of the enzyme carbonic anhydrase.